MarrowCellDLD: a microfluidic method for label-free retrieval of fragile bone marrow-derived cells.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Hematopoietic Stem Cells* ; Bone Marrow*; Humans ; Mice ; Animals ; Microfluidics ; Cell Separation/methods ; Megakaryocytes ; Antigens, CD34 ; Bone Marrow Cells ; Cell Differentiation ; Flow Cytometry

Functional bone marrow studies have focused primarily on hematopoietic progenitors, leaving limited knowledge about other fragile populations, such as bone marrow adipocytes (BMAds) and megakaryocytes. The isolation of these cells is challenging due to rupture susceptibility and large size. We introduce here a label-free cytometry microsystem, MarrowCellDLD, based on deterministic lateral displacement. MarrowCellDLD enables the isolation of large, fragile BM-derived cells based on intrinsic size properties while preserving their viability and functionality. Bone marrow adipocytes, obtained from mouse and human stromal line differentiation, as well as megakaryocytes, from primary human CD34+ hematopoietic stem and progenitor cells, were used for validation. Precise micrometer-range separation cutoffs were adapted for each cell type. Cells were sorted directly in culture media, without pre-labeling steps, and with real-time imaging for quality control. At least 10 ⁶ cells were retrieved intact per sorting round. Our method outperformed two FACS instruments in purity and yield, particularly for large cell size fractions. MarrowCellDLD represents a non-destructive sorting tool for large, fragile BM-derived cells, facilitating the separation of pure populations of BMAds and megakaryocytes to further investigate their physiological and pathological roles.
(© 2023. The Author(s).)

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205321_179086 Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung; PP00P3_183725 Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung; PHRT-308 ETH Personalized Health and Related Technologies Initiative; 56547.1 Innosuisse - Schweizerische Agentur für Innovationsförderung

0 (Antigens, CD34)

Date Created: 20231217 Date Completed: 20231219 Latest Revision: 20231227

20231227

PMC10725893

10.1038/s41598-023-47978-w

38105340