Transcription induces context-dependent remodeling of chromatin architecture during differentiation.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101183755 Publication Model: eCollection Cited Medium: Internet ISSN: 1545-7885 (Electronic) Linking ISSN: 15449173 NLM ISO Abbreviation: PLoS Biol Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science, [2003]-

Chromatin*/genetics ; Chromosomes*/metabolism; Animals ; Mice ; Transcription, Genetic ; Cell Differentiation/genetics ; Genome ; Chromatin Assembly and Disassembly ; CCCTC-Binding Factor/genetics

Metazoan chromosomes are organized into discrete spatial domains (TADs), believed to contribute to the regulation of transcriptional programs. Despite extensive correlation between domain organization and gene activity, a direct mechanistic link is unclear, with perturbation studies often showing little effect. To follow chromatin architecture changes during development, we used Capture Hi-C to interrogate the domains around key differentially expressed genes during mouse thymocyte maturation, uncovering specific remodeling events. Notably, one TAD boundary was broadened to accommodate RNA polymerase elongation past the border, and subdomains were formed around some activated genes without changes in CTCF binding. The ectopic induction of some genes was sufficient to recapitulate domain formation in embryonic stem cells, providing strong evidence that transcription can directly remodel chromatin structure. These results suggest that transcriptional processes drive complex chromosome folding patterns that can be important in certain genomic contexts.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Chahar et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

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0 (Chromatin)
0 (CCCTC-Binding Factor)

Date Created: 20231204 Date Completed: 20231216 Latest Revision: 20231216

20231218

PMC10721200

10.1371/journal.pbio.3002424

38048351