Genome-Wide Identification and Expression Analysis of the Copper Transporter ( COPT / Ctr ) Gene Family in Kandelia obovata , a Typical Mangrove Plant.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Genes, Plant* ; Rhizophoraceae*/genetics; Copper/metabolism ; Copper Transport Proteins/metabolism ; Gene Expression Profiling ; Gene Expression Regulation, Plant ; Genome-Wide Association Study ; Multigene Family ; Phylogeny ; Plant Proteins/metabolism

The copper transporter ( COPT / Ctr ) gene family plays a critical part in maintaining the balance of the metal, and many diverse species depend on COPT to move copper (Cu) across the cell membrane. In Arabidopsis thaliana , Oryza sativa , Medicago sativa , Zea mays , Populus trichocarpa , Vitis vinifera , and Solanum lycopersicum, a genome-wide study of the COPT protein family was performed. To understand the major roles of the COPT gene family in Kandelia obovata ( Ko ), a genome-wide study identified four COPT genes in the Kandelia obovata genome for the first time. The domain and 3D structural variation, phylogenetic tree, chromosomal distributions, gene structure, motif analysis, subcellular localization, cis-regulatory elements, synteny and duplication analysis, and expression profiles in leaves and Cu were all investigated in this research. Structural and sequence investigations show that most KoCOPTs have three transmembrane domains (TMDs). According to phylogenetic research, these KoCOPTs might be divided into two subgroups, just like Populus trichocarpa . KoCOPT gene segmental duplications and positive selection pressure were discovered by universal analysis. According to gene structure and motif analysis, most KoCOPT genes showed consistent exon-intron and motif organization within the same group. In addition, we found five hormones and four stress- and seven light-responsive cis-elements in the KoCOPTs promoters. The expression studies revealed that all four genes changed their expression levels in response to copper (CuCl 2 ) treatments. In summary, our study offers a thorough overview of the Kandelia obovata COPT gene family's expression pattern and functional diversity, making it easier to characterize each KoCOPT gene's function in the future.

Plant Physiol. 2013 May;162(1):180-94. (PMID: 23487432)
Genes (Basel). 2023 Jan 12;14(1):. (PMID: 36672943)
New Phytol. 2011 Oct;192(2):393-404. (PMID: 21692805)
Cell. 1994 Jan 28;76(2):393-402. (PMID: 8293472)
Hortic Res. 2020 May 2;7(1):75. (PMID: 32377365)
PLoS One. 2018 Jul 23;13(7):e0199081. (PMID: 30036360)
Curr Issues Mol Biol. 2022 Nov 13;44(11):5622-5637. (PMID: 36421665)
Hortic Res. 2020 Jun 1;7(1):79. (PMID: 32528691)
Protoplasma. 2019 Jan;256(1):161-170. (PMID: 30043153)
Genes (Basel). 2022 Jul 22;13(8):. (PMID: 35893042)
BMC Plant Biol. 2011 Apr 21;11:69. (PMID: 21510855)
Chemosphere. 2013 Jan;90(2):150-7. (PMID: 22795073)
Curr Opin Chem Biol. 2002 Apr;6(2):171-80. (PMID: 12039001)
Plant Cell Physiol. 2012 Nov;53(11):1866-80. (PMID: 22952251)
Tree Physiol. 2022 May 9;42(5):1016-1028. (PMID: 34918132)
Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4237-42. (PMID: 19240214)
New Phytol. 2009 Jun;182(4):799-816. (PMID: 19402880)
Front Plant Sci. 2023 Sep 15;14:1242836. (PMID: 37780503)
Planta. 2014 Jul;240(1):91-101. (PMID: 24691572)
New Phytol. 2014 Apr;202(1):19-34. (PMID: 24251960)
Int J Biol Macromol. 2021 Jun 30;181:644-652. (PMID: 33798576)
Trop Life Sci Res. 2015 Apr;26(1):53-65. (PMID: 26019747)
Trends Microbiol. 1999 Dec;7(12):500-5. (PMID: 10603486)
iScience. 2021 Nov 30;25(1):103547. (PMID: 34988398)
Plant Mol Biol. 2003 Mar;51(4):577-87. (PMID: 12650623)
Plant Physiol. 2008 Oct;148(2):993-1003. (PMID: 18715958)
J Hazard Mater. 2010 Oct 15;182(1-3):848-54. (PMID: 20667653)
J Biol Inorg Chem. 2010 Jan;15(1):29-36. (PMID: 19798519)
Microbiology (Reading). 2011 Apr;157(Pt 4):1021-1031. (PMID: 21273250)
Int J Mol Sci. 2022 Jun 10;23(12):. (PMID: 35742940)
Biology (Basel). 2022 Aug 08;11(8):. (PMID: 36009819)
Plant Cell Physiol. 2013 Aug;54(8):1378-90. (PMID: 23766354)
Environ Geochem Health. 2019 Feb;41(1):135-148. (PMID: 29987496)
FEBS Lett. 1996 May 13;386(1):1-4. (PMID: 8635592)
Bioinformatics. 1998;14(4):378-9. (PMID: 9632836)
Mar Pollut Bull. 2017 Nov 30;124(2):1089-1095. (PMID: 28442201)
Front Plant Sci. 2023 Mar 01;14:1112354. (PMID: 36938021)
Sci Total Environ. 2020 Feb 10;703:134807. (PMID: 31744692)
Sci Rep. 2022 Aug 23;12(1):14389. (PMID: 35999231)
Plant Physiol Biochem. 2015 Dec;97:451-60. (PMID: 26581045)
J Biol Chem. 2004 Apr 9;279(15):15348-55. (PMID: 14726516)

2018YFA0902500 National Key R&D Program of China; 42376219 National Natural Science Foundation of China; 41706137 National Natural Science Foundation of China; No. OOST2021- 07 CAS Key Laboratory of Science and Technology on Operational Oceanography; Guangdong Province, 2020JGXM094 Graduate Education Innovation Project

Keywords: 3D structures; Kandelia obovata; copper stress; copper transporter; qRT-PCR

789U1901C5 (Copper)
0 (Copper Transport Proteins)
0 (Plant Proteins)

Date Created: 20231114 Date Completed: 20231127 Latest Revision: 20231127

20231215

PMC10648262

10.3390/ijms242115579

37958561