Polyphenol Profiling by LC QTOF/ESI-MS and Biological Activity of Purple Passion Fruit Epicarp Extract.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, c1995-

Polyphenols*/pharmacology ; Polyphenols*/analysis ; Antioxidants*/pharmacology; Fruit/chemistry ; alpha-Glucosidases ; Anthocyanins ; Cyclooxygenase 2 ; Plant Extracts/pharmacology ; alpha-Amylases

A polyphenolic preparation in the form of the passion fruit epicarp extract was analyzed to identify and quantify the polyphenolic compounds using LC QTOF/ESI-MS and UPLC-PDA-FL. The analyzed parameters included antidiabetic activity (α-amylase, α-glucosidase, and pancreatic lipase), inhibitory activity toward cholinesterase (AChE, BuChE), anti-inflammatory activity (COX-1, COX-2, 15-LOX) and antioxidant activity based on ORAC and ABTS. The polyphenolic preparation of the passion fruit epicarp extract contained 51 polyphenolic compounds representing five groups-flavones (25 compounds; 52% of total polyphenolic), flavonols (8; 16%), flavan-3-ols (6; 7%), phenolic acids (4; 3%), and anthocyanins (7; 21%), with derivatives of luteolin (13 derivatives) and apigenin (8 derivatives) as dominant compounds. The preparation was characterized by an antioxidant activity of 160.7 (ORAC) and 1004.4 mmol Trolox/100 mL (ABTS ^+o ). The inhibitory activity toward α-amylase, α-glucosidase, and pancreatic lipase reached IC 50 of 7.99, 12.80, and 0.42, respectively. The inhibition of cholinesterases (IC 50 ) was 18.29 for AChE and 14.22 for BuChE. Anti-inflammatory activity as IC 50 was 6.0 for COX-1, 0.9 for COX-2, and 4.9 for 15-LOX. Food enriched with passion fruit epicarp extract has a potentially therapeutic effect.

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Keywords: Passiflora edulis Sims; chromatography; in vitro enzyme inhibition effect; passion epicarp fruits extract

0 (Polyphenols)
0 (Antioxidants)
EC 3.2.1.20 (alpha-Glucosidases)
0 (Anthocyanins)
28752-68-3 (2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid)
EC 1.14.99.1 (Cyclooxygenase 2)
0 (Plant Extracts)
EC 3.2.1.1 (alpha-Amylases)

Date Created: 20230928 Date Completed: 20231004 Latest Revision: 20231004

20231215

PMC10535944

10.3390/molecules28186711

37764487