The potential use of glycosyl-transferase inhibitors for targeted reduction of S. mutans biofilms in dental materials.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Biofilms* ; Dental Caries*; Humans ; Streptococcus mutans ; Polysaccharides/pharmacology ; Glucosyltransferases ; Dental Materials

Streptococcus mutans is the primary oral caries-forming bacteria, adept at producing "sticky" biofilms via the synthesis of insoluble extracellular polysaccharides (EPS), catalyzed by glucosyltransferases (GTFs). To circumvent the use of broad-spectrum antibiotics to combat these bacteria, this study sought to modify existing EPS-targeting small molecules with the ultimate goal of producing anti-biofilm polymer surfaces specifically targeting S. mutans. To achieve this, a known GTF inhibitor (G43) was modified with methoxy or tetraethyleneglycol substitutions in different positions (nine derivatives, tested at 50-µM) to pinpoint potential sites for future methacrylate functionalization, and then assessed against single-species S. mutans biofilms. As expected, the compounds did not diminish the bacterial viability. In general, the compounds with methoxy substitution were not effective in reducing EPS formation, whereas the tetraethyleneglycol substitution (G43-C3-TEG) led to a decrease in the concentration of insoluble EPS, although the effect is less pronounced than for the parent G43. This aligns with the reduced GTF-C activity observed at different concentrations of G43-C3-TEG, as well as the consequent decrease in EPS formation, and notable structural changes. In summary, this study determined that G43-C3-TEG is non-bactericidal and can selectively reduce the biofilm formation, by decreasing the production of EPS. This molecule will serve to functionalize surfaces of materials to be tested in future research.
(© 2023. The Author(s).)

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R01 DE022350 United States DE NIDCR NIH HHS; R35-DE029083 United States DE NIDCR NIH HHS; K02 DE025280 United States DE NIDCR NIH HHS; R01 DE028757 United States DE NIDCR NIH HHS

0 (Polysaccharides)
EC 2.4.1.- (Glucosyltransferases)
0 (Dental Materials)

Date Created: 20230723 Date Completed: 20230725 Latest Revision: 20240718

20240718

PMC10363545

10.1038/s41598-023-39125-2

37482546