Knockdown of DOM/Tip60 Complex Subunits Impairs Male Meiosis of Drosophila melanogaster .

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101600052 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4409 (Electronic) Linking ISSN: 20734409 NLM ISO Abbreviation: Cells Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI

Drosophila melanogaster*/metabolism ; Drosophila Proteins*/genetics ; Drosophila Proteins*/metabolism; Animals ; Humans ; Male ; Adenosine Triphosphate/metabolism ; Chromatin/metabolism ; Drosophila/metabolism ; Histone Acetyltransferases/genetics ; Histone Acetyltransferases/metabolism ; Meiosis/genetics ; Nucleosomes/metabolism

ATP-dependent chromatin remodeling complexes are involved in nucleosome sliding and eviction and/or the incorporation of histone variants into chromatin to facilitate several cellular and biological processes, including DNA transcription, replication and repair. The DOM/TIP60 chromatin remodeling complex of Drosophila melanogaster contains 18 subunits, including the DOMINO (DOM), an ATPase that catalyzes the exchange of the canonical H2A with its variant (H2A.V), and TIP60, a lysine-acetyltransferase that acetylates H4, H2A and H2A.V histones. In recent decades, experimental evidence has shown that ATP-dependent chromatin remodeling factors, in addition to their role in chromatin organization, have a functional relevance in cell division. In particular, emerging studies suggested the direct roles of ATP-dependent chromatin remodeling complex subunits in controlling mitosis and cytokinesis in both humans and D. melanogaster. However, little is known about their possible involvement during meiosis. The results of this work show that the knockdown of 12 of DOM/TIP60 complex subunits generates cell division defects that, in turn, cause total/partial sterility in Drosophila males, providing new insights into the functions of chromatin remodelers in cell division control during gametogenesis.

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Keywords: ATPase; DOMINO; Drosophila male meiosis; TIP60; cell division; chromatin remodeling; epigenetics

8L70Q75FXE (Adenosine Triphosphate)
0 (Chromatin)
0 (Drosophila Proteins)
EC 2.3.1.48 (Histone Acetyltransferases)
0 (Nucleosomes)
EC 2.3.1.48 (Tip60 protein, Drosophila)
0 (dom protein, Drosophila)

Date Created: 20230706 Date Completed: 20230710 Latest Revision: 20230718

20230718

PMC10216235

10.3390/cells12101348

37408183