Effects of Four Extraction Methods on Structure and In Vitro Fermentation Characteristics of Soluble Dietary Fiber from Rape Bee Pollen.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, c1995-

Fatty Acids, Volatile*/analysis ; Bacteria*; Animals ; Bees ; Fermentation ; Phenols/chemistry ; Pollen/chemistry ; Bacteroidetes ; Dietary Fiber/analysis

In this study, soluble dietary fibers (SDFs) were extracted from rape bee pollen using four methods including acid extraction (AC), alkali extraction (AL), cellulase extraction (CL) and complex enzyme extraction (CE). The effects of different extraction methods on the structure of SDFs and in vitro fermentation characteristics were further investigated. The results showed that the four extraction methods significantly affected the monosaccharide composition molar ratio, molecular weight, surface microstructure and phenolic compounds content, but showed little effect on the typical functional groups and crystal structure. In addition, all SDFs decreased the Firmicutes / Bacteroidota ratio, promoted the growth of beneficial bacteria such as Bacteroides , Parabacteroides and Phascolarctobacterium , inhibited the growth of pathogenic bacteria such as Escherichia-Shigella , and increased the total short-chain fatty acids (SCFAs) concentrations by 1.63-2.45 times, suggesting that the bee pollen SDFs had a positive regulation on gut microbiota. Notably, the SDF obtained by CE exhibited the largest molecular weight, a relatively loose structure, higher extraction yield and phenolic compounds content and the highest SCFA concentration. Overall, our results indicated that CE was an appropriate extraction method of high-quality bee pollen SDF.

Nat Protoc. 2019 Apr;14(4):991-1014. (PMID: 30886367)
Foods. 2022 Jul 06;11(14):. (PMID: 35885237)
Crit Rev Food Sci Nutr. 2022 Mar 23;:1-18. (PMID: 35319324)
Pflugers Arch. 2019 Dec;471(11-12):1441-1453. (PMID: 31728701)
Gut Microbes. 2021 Jan-Dec;13(1):1-21. (PMID: 33525961)
Front Nutr. 2023 Feb 23;10:1064662. (PMID: 36908912)
Front Microbiol. 2021 Apr 12;12:658292. (PMID: 33912150)
Cell Host Microbe. 2018 Jun 13;23(6):705-715. (PMID: 29902436)
J Environ Sci Health B. 2016;51(3):176-81. (PMID: 26674447)
Microbiologyopen. 2021 Mar;10(2):e1181. (PMID: 33970546)
Pharmacol Res. 2023 Mar;189:106687. (PMID: 36746362)
Food Res Int. 2020 Oct;136:109497. (PMID: 32846578)
Trends Biotechnol. 2015 Sep;33(9):496-503. (PMID: 26210164)
Environ Microbiol. 2022 Sep;24(9):3861-3881. (PMID: 35233904)
Nat Commun. 2015 Jun 23;6:7489. (PMID: 26102296)
Front Microbiol. 2020 Oct 27;11:575586. (PMID: 33193190)
J Mol Med (Berl). 2017 Jan;95(1):1-8. (PMID: 27900395)
Molecules. 2021 Dec 26;27(1):. (PMID: 35011349)
Nat Commun. 2019 May 3;10(1):2043. (PMID: 31053724)
Cell. 2020 Mar 19;180(6):1067-1080.e16. (PMID: 32160527)
Foods. 2022 Jul 21;11(14):. (PMID: 35885404)
Plant Foods Hum Nutr. 2016 Mar;71(1):1-12. (PMID: 26847187)
Food Chem X. 2022 Apr 01;14:100298. (PMID: 35399582)
Foods. 2022 Oct 31;11(21):. (PMID: 36360070)
Carbohydr Polym. 2021 Jan 1;251:117041. (PMID: 33142599)
Molecules. 2022 Nov 10;27(22):. (PMID: 36431835)
Int J Mol Sci. 2022 Sep 24;23(19):. (PMID: 36232580)
J Food Sci. 2020 Jun;85(6):1668-1674. (PMID: 32458493)
Neurogastroenterol Motil. 2016 Aug;28(8):1233-40. (PMID: 27027909)
Int J Biol Macromol. 2021 Dec 1;192:398-406. (PMID: 34571128)
FEMS Microbiol Lett. 2022 Aug 29;369(1):. (PMID: 35945336)
J Anim Sci Biotechnol. 2019 May 24;10:45. (PMID: 31149336)
J Am Soc Nephrol. 2022 Dec;33(12):2276-2292. (PMID: 36041791)
Food Res Int. 2022 Jul;157:111489. (PMID: 35761712)
PLoS One. 2020 Sep 30;15(9):e0239428. (PMID: 32997697)
FEMS Microbiol Rev. 2021 Mar 16;45(2):. (PMID: 33026073)
J Food Biochem. 2020 Dec;44(12):e13524. (PMID: 33073381)
mSystems. 2020 Oct 27;5(5):. (PMID: 33109749)
Microorganisms. 2020 Apr 15;8(4):. (PMID: 32326636)
Int J Biol Macromol. 2022 Dec 31;223(Pt A):1196-1207. (PMID: 36347374)
Int J Biol Macromol. 2022 Dec 1;222(Pt A):610-619. (PMID: 36167101)
Food Chem. 2023 Mar 30;405(Pt A):134800. (PMID: 36347200)
Food Chem. 2023 May 30;409:135342. (PMID: 36586262)
Microbiologyopen. 2020 Oct;9(10):e1111. (PMID: 32856395)
Food Funct. 2022 Sep 22;13(18):9443-9458. (PMID: 35972431)

No.2022JJ5410 Hunan Provincial Department of Science and Technology

Keywords: bee pollen; extraction methods; in vitro fermentation characteristics; soluble dietary fiber; structure

0 (Fatty Acids, Volatile)
0 (Phenols)
0 (Dietary Fiber)

Date Created: 20230628 Date Completed: 20230629 Latest Revision: 20230701

20240829

PMC10301365

10.3390/molecules28124800

37375355