Genome-Wide Identification and Expression Analysis of Dendrocalamus farinosus CCoAOMT Gene Family and the Role of DfCCoAOMT14 Involved in Lignin Synthesis.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Poaceae*/genetics ; Methyltransferases*/genetics ; Genes, Plant* ; Lignin*/biosynthesis ; Lignin*/genetics ; Plants, Genetically Modified*/genetics ; Gene Expression Regulation, Plant*/genetics; Drought Resistance/genetics ; Genome-Wide Association Study

As the main component of plant cell walls, lignin can not only provide mechanical strength and physical defense for plants, but can also be an important indicator affecting the properties and quality of wood and bamboo. Dendrocalamus farinosus is an important economic bamboo species for both shoots and timber in southwest China, with the advantages of fast growth, high yield and slender fiber. Caffeoyl-coenzyme A-O-methyltransferase (CCoAOMT) is a key rate-limiting enzyme in the lignin biosynthesis pathway, but little is known about it in D. farinosus. Here, a total of 17 DfCCoAOMT genes were identified based on the D. farinosus whole genome. DfCCoAOMT1/14/15/16 were homologs of AtCCoAOMT1 . DfCCoAOMT6/9/14/15/16 were highly expressed in stems of D. farinosus ; this is consistent with the trend of lignin accumulation during bamboo shoot elongation, especially DfCCoAOMT14 . The analysis of promoter cis-acting elements suggested that DfCCoAOMTs might be important for photosynthesis, ABA/MeJA responses, drought stress and lignin synthesis. We then confirmed that the expression levels of DfCCoAOMT2/5/6/8/9/14/15 were regulated by ABA/MeJA signaling. In addition, overexpression of DfCCoAOMT14 in transgenic plants significantly increased the lignin content, xylem thickness and drought resistance of plants. Our findings revealed that DfCCoAOMT14 can be a candidate gene that is involved in the drought response and lignin synthesis pathway in plants, which could contribute to the genetic improvement of many important traits in D. farinosus and other species.

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2021YFD2200504_1 and 2021YFD2200505_2 National Key R&D Program of China; 2022NSFSC0093 and 2021YFYZ0006 Science and Technology Project of Sichuan Province, China; 22zx7114 Ph.D. Foundation from Southwest University of Science and Technology

Keywords: CCoAOMT gene; Dendrocalamus farinosus; drought response; genome-wide identification; lignin synthesis

EC 2.1.1.104 (caffeoyl-CoA O-methyltransferase)
EC 2.1.1.- (Methyltransferases)
9005-53-2 (Lignin)

Date Created: 20230527 Date Completed: 20230529 Latest Revision: 20240402

20240403

PMC10219071

10.3390/ijms24108965

37240316