Effects of Blue Light on Puberty and Ovary in Female Rats

Publisher: Galenos Yayınevi Country of Publication: Turkey NLM ID: 101519456 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1308-5735 (Electronic) NLM ISO Abbreviation: J Clin Res Pediatr Endocrinol Subsets: MEDLINE

Publication: Istanbul : Galenos Yayınevi
Original Publication: Istanbul : Turkish Pediatric Endocrinology and Diabetes Society

Melatonin* ; Polycystic Ovary Syndrome*; Rats ; Female ; Humans ; Animals ; Leptin ; Rats, Sprague-Dawley ; Luteinizing Hormone ; Follicle Stimulating Hormone ; Estradiol ; Puberty ; Testosterone ; Dehydroepiandrosterone

Objective: This study was designed to examine the effect of blue light exposure and exposure time on puberty in an animal model.
Methods: Eighteen 21-day-old female Sprague Dawley rats were divided into three equal groups which were: control group (CG); blue light-6 hours (BL-6); and blue light-12 hours (BL-12). CG rats were maintained with 12/12-hour light-dark cycles. The animals in BL-6 and BL-12 were exposed to blue light of wavelength 450-470 nm and intensity of 0.03 uW/cm ² for 6 and 12 hours, respectively. Exposure to blue light continued until the first signs of puberty. Serum follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol, testosterone, dehydroepiandrosterone sulfate (DHEA-S), leptin and melatonin were measured. Subsequently the ovaries and uterus were examined histomorphologically.
Results: The median day of puberty start was 38, 32 and 30 for the CG, BL-6, and BL-12 groups, respectively (p=0.001). FSH, testosterone, DHEA-S, and leptin concentrations of all groups were similar. However, LH and estradiol concentrations in BL-6 were higher compared to CG (p=0.02). There was a negative correlation between blue light exposure, exposure time, and melatonin concentrations (r=-0.537, p=0.048). Ovarian tissue was compatible with puberty in all groups. As blue light exposure time increased, capillary dilatation and edema in the ovarian tissue increased. Prolonged exposure was associated with polycystic ovary-like (PCO) morphological changes and apoptosis in granulosa cells.
Conclusion: These results suggest that exposure to blue light and the duration of exposure induced earlier puberty in female rats. As the duration of blue light exposure increased, PCO-like inflammation, and apoptosis were detected in the ovaries.
Competing Interests: Conflict of interest: None declared
(©Copyright 2023 by Turkish Society for Pediatric Endocrinology and Diabetes / The Journal of Clinical Research in Pediatric Endocrinology published by Galenos Publishing House.)

Endocrinology. 2014 Oct;155(10):3934-44. (PMID: 25051447)
Physiol Behav. 2022 Jan 1;243:113641. (PMID: 34748861)
J Clin Endocrinol Metab. 2015 Nov;100(11):4067-73. (PMID: 26301944)
Middle East Afr J Ophthalmol. 2020 Oct 30;27(3):177-181. (PMID: 33488015)
J Clin Endocrinol Metab. 1991 Oct;73(4):793-6. (PMID: 1909703)
Front Neurosci. 2013 Apr 16;7:60. (PMID: 23596387)
Ital J Pediatr. 2020 Nov 4;46(1):165. (PMID: 33148304)
J Clin Invest. 1997 Feb 1;99(3):391-5. (PMID: 9022071)
Brain Res Brain Res Rev. 2005 Dec 15;50(2):213-28. (PMID: 16216333)
Sleep Sci. 2019 Jul-Sep;12(3):171-177. (PMID: 31890092)
J Biol Rhythms. 2013 Aug;28(4):262-71. (PMID: 23929553)
Sci Rep. 2017 Apr 12;7:46381. (PMID: 28401948)
Semin Reprod Med. 2002 May;20(2):139-44. (PMID: 12087499)
Endocr Connect. 2022 Feb 14;11(2):. (PMID: 35029543)
Chronobiol Int. 2019 Feb;36(2):151-170. (PMID: 30311830)
BMJ Open. 2017 Oct 24;7(10):e014585. (PMID: 29070636)
J Clin Endocrinol Metab. 2005 Mar;90(3):1311-6. (PMID: 15585546)
JAMA Pediatr. 2022 Apr 01;176(4):400-409. (PMID: 35040870)
Front Public Health. 2015 Oct 13;3:233. (PMID: 26528465)
Exp Gerontol. 2001 Jul;36(7):1083-100. (PMID: 11404053)
Animals (Basel). 2019 Sep 30;9(10):. (PMID: 31575048)
Int J Epidemiol. 2013 Apr;42(2):590-600. (PMID: 23569194)
Neuroendocrinology. 1982 Feb;34(2):112-6. (PMID: 6122168)
Dev Cogn Neurosci. 2022 Oct;57:101150. (PMID: 36084446)
Toxicol Pathol. 2015 Aug;43(6):776-93. (PMID: 25739587)
Endocrinology. 2007 Aug;148(8):3781-91. (PMID: 17495003)
J Pineal Res. 2001 May;30(4):227-33. (PMID: 11339512)
J Pineal Res. 2012 Aug;53(1):47-59. (PMID: 22017511)
Sleep. 2013 Aug 01;36(8):1239-46. (PMID: 23904684)
Science. 1999 Apr 16;284(5413):505-7. (PMID: 10205062)
Biomed Res Int. 2015;2015:943694. (PMID: 26064969)
J Clin Endocrinol Metab. 2019 Aug 1;104(8):3525-3534. (PMID: 30888398)
Eye (Lond). 1999 Aug;13 ( Pt 4):577-83. (PMID: 10692935)
Dev Psychol. 2015 Jun;51(6):816-822. (PMID: 25915592)
J Clin Res Pediatr Endocrinol. 2022 May 31;14(3):302-307. (PMID: 35633642)
Endocr Rev. 2020 Jul 1;41(4):. (PMID: 32310267)
Sleep Med Rev. 2005 Feb;9(1):25-39. (PMID: 15649736)
Sci China Life Sci. 2020 Jan;63(1):18-58. (PMID: 31813094)
J Pineal Res. 1999 Nov;27(4):193-201. (PMID: 10551766)
Physiol Rep. 2018 Mar;6(5):. (PMID: 29504270)
Sci Rep. 2022 Feb 7;12(1):2015. (PMID: 35132065)
Front Microbiol. 2020 Jan 21;10:3145. (PMID: 32038578)
Physiol Rep. 2018 Dec;6(24):e13942. (PMID: 30556352)

Keywords: melatonin; rat; Blue light (470 nm); early puberty onset; apoptosis

0 (Leptin)
JL5DK93RCL (Melatonin)
9002-67-9 (Luteinizing Hormone)
9002-68-0 (Follicle Stimulating Hormone)
4TI98Z838E (Estradiol)
3XMK78S47O (Testosterone)
459AG36T1B (Dehydroepiandrosterone)

Date Created: 20230522 Date Completed: 20231128 Latest Revision: 20231202

20250114

PMC10683537

10.4274/jcrpe.galenos.2023.2022-12-1

37212628