Size-Controlled DNA Tile Self-Assembly Nanostructures Through Caveolae-Mediated Endocytosis for Signal-Amplified Imaging of MicroRNAs in Living Cells.

Publisher: WILEY-VCH Country of Publication: Germany NLM ID: 101664569 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2198-3844 (Electronic) Linking ISSN: 21983844 NLM ISO Abbreviation: Adv Sci (Weinh) Subsets: MEDLINE

Original Publication: Weinheim : WILEY-VCH, [2014]-

MicroRNAs*/genetics ; Nanostructures*/chemistry; Caveolae ; DNA/chemistry ; Endocytosis

Signal-amplified imaging of microRNAs (miRNAs) is a promising strategy at the single-cell level because liquid biopsy fails to reflect real-time dynamic miRNA levels. However, the internalization pathways for available conventional vectors predominantly involve endo-lysosomes, showing nonideal cytoplasmic delivery efficiency. In this study, size-controlled 9-tile nanoarrays are designed and constructed by integrating catalytic hairpin assembly (CHA) with DNA tile self-assembly technology to achieve caveolae-mediated endocytosis for the amplified imaging of miRNAs in a complex intracellular environment. Compared with classical CHA, the 9-tile nanoarrays possess high sensitivity and specificity for miRNAs, achieve excellent internalization efficiency by caveolar endocytosis, bypassing lysosomal traps, and exhibit more powerful signal-amplified imaging of intracellular miRNAs. Because of their excellent safety, physiological stability, and highly efficient cytoplasmic delivery, the 9-tile nanoarrays can realize real-time amplified monitoring of miRNAs in various tumor and identical cells of different periods, and imaging effects are consistent with the actual expression levels of miRNAs, ultimately demonstrating their feasibility and capacity. This strategy provides a high-potential delivery pathway for cell imaging and targeted delivery, simultaneously offering a meaningful reference for the application of DNA tile self-assembly technology in relevant fundamental research and medical diagnostics.
(© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.)

J Cell Physiol. 2019 Jul;234(7):9927-9942. (PMID: 30536403)
Nature. 2017 Feb 23;542(7642):450-455. (PMID: 28199304)
Science. 2015 Feb 20;347(6224):1260901. (PMID: 25700524)
Acc Chem Res. 2016 Jun 21;49(6):1139-48. (PMID: 27203418)
Biosens Bioelectron. 2022 Feb 15;198:113821. (PMID: 34840013)
Nucleic Acids Res. 2019 Nov 18;47(20):10489-10505. (PMID: 31287874)
Adv Drug Deliv Rev. 2015 Aug 30;91:92-108. (PMID: 25579057)
Biomaterials. 2018 Mar;157:98-106. (PMID: 29258013)
Acta Pharmacol Sin. 2010 Jul;31(7):867-73. (PMID: 20581857)
J Control Release. 2022 Dec;352:637-651. (PMID: 36349616)
J Cell Sci. 2003 Dec 1;116(Pt 23):4707-14. (PMID: 14600257)
Biol Rev Camb Philos Soc. 2016 May;91(2):409-28. (PMID: 25631495)
Chem Sci. 2020 Jul 23;11(32):8482-8488. (PMID: 34123108)
Cancer Cell. 2010 Sep 14;18(3):282-93. (PMID: 20832755)
Angew Chem Int Ed Engl. 2017 Dec 11;56(50):16023-16027. (PMID: 29076273)
J Exp Clin Cancer Res. 2018 Nov 6;37(1):268. (PMID: 30400960)
Signal Transduct Target Ther. 2021 Oct 8;6(1):351. (PMID: 34620843)
Genome Med. 2013 Dec 30;5(12):111. (PMID: 24373327)
Biosens Bioelectron. 2022 Feb 1;197:113809. (PMID: 34814030)
J Clin Invest. 2016 Jan;126(1):349-64. (PMID: 26657862)
Mol Aspects Med. 2020 Apr;72:100832. (PMID: 31767382)
Mol Cancer. 2020 Feb 15;19(1):29. (PMID: 32059676)
Cancer Lett. 2021 Mar 31;501:66-82. (PMID: 33385486)
Nat Biotechnol. 2013 Jul;31(7):638-46. (PMID: 23792630)
Adv Drug Deliv Rev. 2010 Apr 30;62(6):617-25. (PMID: 20230866)
Eur J Cancer. 2011 Nov;47(16):2479-90. (PMID: 21775132)
Trends Mol Med. 2014 Aug;20(8):460-9. (PMID: 25027972)
Cell. 2012 Apr 27;149(3):515-24. (PMID: 22541426)
Nature. 2016 Oct 12;538(7624):183-192. (PMID: 27734871)
Acc Chem Res. 2017 Apr 18;50(4):1059-1068. (PMID: 28355077)
Traffic. 2003 Nov;4(11):724-38. (PMID: 14617356)
Nano Lett. 2007 Oct;7(10):3056-64. (PMID: 17887715)
Adv Healthc Mater. 2018 Jan;7(1):. (PMID: 28809092)
Biosens Bioelectron. 2023 Feb 15;222:114932. (PMID: 36462429)
Saudi J Biol Sci. 2019 May;26(4):777-781. (PMID: 31049003)
Annu Rev Pathol. 2009;4:199-227. (PMID: 18817506)
J Hepatol. 2012 Jan;56(1):167-75. (PMID: 21749846)
Acta Pharm Sin B. 2021 Apr;11(4):961-977. (PMID: 33996409)
J Hepatol. 2010 Jul;53(1):98-107. (PMID: 20447717)
Anal Chem. 2022 Feb 15;94(6):2693-2698. (PMID: 35119262)
J Hematol Oncol. 2014 Jan 10;7:6. (PMID: 24405714)
Physiol Rev. 2011 Jul;91(3):827-87. (PMID: 21742789)
J Hematol Oncol. 2020 Mar 28;13(1):25. (PMID: 32222150)
Biomaterials. 2015;53:453-63. (PMID: 25890742)
Annu Rev Biochem. 2010;79:65-87. (PMID: 20222824)
J Cell Biol. 2015 Apr 13;209(1):163-80. (PMID: 25847537)
Bioact Mater. 2021 Feb 27;6(9):2946-2955. (PMID: 33732965)
Clin Epigenetics. 2018 Apr 23;10:59. (PMID: 29713393)
Biomaterials. 2008 Aug;29(23):3367-75. (PMID: 18485474)
Adv Sci (Weinh). 2017 Sep 05;4(11):1700324. (PMID: 29201626)
Nat Commun. 2018 Oct 17;9(1):4319. (PMID: 30333487)
Nat Biotechnol. 2007 Mar;25(3):327-37. (PMID: 17334358)
Chem Rev. 2020 Sep 9;120(17):9420-9481. (PMID: 32672036)
Small. 2019 Oct;15(42):e1902989. (PMID: 31523917)
Gastroenterology. 2009 Jun;136(7):2304-2315.e1-4. (PMID: 19272382)
Adv Sci (Weinh). 2023 Jul;10(21):e2300614. (PMID: 37189216)
Trends Cell Biol. 2016 Mar;26(3):177-189. (PMID: 26653791)
Cell Prolif. 2015 Oct;48(5):511-6. (PMID: 26202219)
Mol Cancer. 2010 Nov 26;9:306. (PMID: 21110877)
Angew Chem Int Ed Engl. 2022 Dec 19;61(51):e202214230. (PMID: 36383756)
Chem Rev. 2019 May 22;119(10):6459-6506. (PMID: 29465222)
Adv Drug Deliv Rev. 2019 Jul;147:2-21. (PMID: 30769047)
Adv Drug Deliv Rev. 2019 Jul;147:22-28. (PMID: 30769045)
Small. 2020 Mar;16(11):e1906144. (PMID: 32037728)
ACS Nano. 2020 Jan 28;14(1):289-302. (PMID: 31869202)
Annu Rev Biomed Eng. 2012;14:1-16. (PMID: 22524388)
Acta Biomater. 2015 Oct;25:184-93. (PMID: 26169933)
Chem Rev. 2019 May 22;119(10):6273-6289. (PMID: 29911864)
J Control Release. 2012 Mar 28;158(3):413-23. (PMID: 22226779)
J Hematol Oncol. 2010 Oct 06;3:37. (PMID: 20925959)

82272440 National Natural Science Foundation of China; 82060386 National Natural Science Foundation of China; 81860373 National Natural Science Foundation of China; 2021YFE0202000 National Key R&D Program of China; 823CXTD376 Hainan Provincial Natural Science Foundation of China; ZDKJ2021029 Hainan Province Science and Technology Special Fund; ZDYF2021SHFZ068 Hainan Province Science and Technology Special Fund; 2019-I2M-5-023 CAMS Innovation Fund for Medical Sciences; Qhyb2021-56 Education Department of Hainan Province; Qhys2022-274 Education Department of Hainan Province; Qhys2021-341 Education Department of Hainan Province; Qhys2021-342 Education Department of Hainan Province; and Qhys2021-344 Education Department of Hainan Province

Keywords: DNA nanostructures; DNA tile self-assembly technology; caveolae-mediated endocytosis; living cells; microRNAs; signal-amplified imaging; size controlled

0 (MicroRNAs)
9007-49-2 (DNA)

Date Created: 20230515 Date Completed: 20230731 Latest Revision: 20240930

20240930

PMC10375201

10.1002/advs.202300614

37189216