L-Lactate treatment by photosynthetic cyanobacteria expressing heterogeneous L-lactate dehydrogenase.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

L-Lactate Dehydrogenase*/genetics ; L-Lactate Dehydrogenase*/metabolism ; Synechococcus*/genetics ; Synechococcus*/metabolism; Animals ; Malates/metabolism ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Ketoglutaric Acids/metabolism ; Lactic Acid/metabolism ; Succinates/metabolism

L-Lactate is a major waste compound in cultured animal cells. To develop a sustainable animal cell culture system, we aimed to study the consumption of L-lactate using a photosynthetic microorganism. As genes involved in L-lactate utilization were not found in most cyanobacteria and microalgae, we introduced the NAD-independent L-lactate dehydrogenase gene from Escherichia coli (lldD) into Synechococcus sp. PCC 7002. The lldD-expressing strain consumed L-lactate added to basal medium. This consumption was accelerated by expression of a lactate permease gene from E. coli (lldP) and an increase in culture temperature. Intracellular levels of acetyl-CoA, citrate, 2-oxoglutarate, succinate, and malate, and extracellular levels of 2-oxoglutarate, succinate, and malate, increased during L-lactate utilization, suggesting that the metabolic flux from L-lactate was distributed toward the tricarboxylic acid cycle. This study provides a perspective on L-lactate treatment by photosynthetic microorganisms, which would increase the feasibility of animal cell culture industries.
(© 2023. The Author(s).)

Biotechnol Prog. 2022 May;38(3):e3239. (PMID: 35073462)
Ann N Y Acad Sci. 2014 Nov;1328:29-33. (PMID: 25376889)
Arch Microbiol. 2022 Sep 12;204(10):615. (PMID: 36094577)
J Bacteriol. 2012 May;194(10):2687-92. (PMID: 22408166)
ACS Synth Biol. 2020 Feb 21;9(2):260-268. (PMID: 32004431)
Trends Microbiol. 2014 Oct;22(10):589-99. (PMID: 24950803)
Microbiology (Reading). 2012 Dec;158(Pt 12):3032-3043. (PMID: 23038809)
Reprod Med Biol. 2017 Mar 21;16(2):99-117. (PMID: 29259457)
Arch Microbiol. 2021 Nov;203(9):5525-5532. (PMID: 34426852)
Mar Biotechnol (NY). 2021 Apr;23(2):331-341. (PMID: 33713238)
PLoS One. 2017 Jan 12;12(1):e0168796. (PMID: 28081236)
FEMS Microbiol Ecol. 2007 Jul;61(1):74-84. (PMID: 17466025)
Int J Mol Sci. 2020 Dec 31;22(1):. (PMID: 33396191)
Appl Environ Microbiol. 2015 Sep;81(18):6210-22. (PMID: 26150450)
Proc Natl Acad Sci U S A. 2011 Mar 15;108(11):4352-7. (PMID: 21368207)
Biotechnol Prog. 2020 Mar;36(2):e2941. (PMID: 31756286)
Appl Environ Microbiol. 2014 Dec;80(23):7134-41. (PMID: 25217009)
Front Microbiol. 2011 Dec 29;2:261. (PMID: 22232617)
Nat Biotechnol. 2004 Nov;22(11):1393-8. (PMID: 15529164)
Commun Biol. 2021 Apr 9;4(1):450. (PMID: 33837247)
Front Microbiol. 2016 Apr 05;7:475. (PMID: 27092129)
Nat Biotechnol. 2018 Dec 6;36(12):1136-1145. (PMID: 30520869)
ACS Synth Biol. 2019 Dec 20;8(12):2701-2709. (PMID: 31653173)
Appl Environ Microbiol. 2005 Oct;71(10):5920-8. (PMID: 16204505)
Front Plant Sci. 2020 Jan 31;10:1700. (PMID: 32117327)
Sci Rep. 2015 May 05;5:9777. (PMID: 25940225)
J Exp Bot. 2013 Jul;64(10):2943-54. (PMID: 23658429)
J Biotechnol. 1996 May 15;46(3):161-85. (PMID: 8672289)
J Exp Bot. 2020 Mar 25;71(6):2018-2027. (PMID: 31863668)
Nucleic Acids Res. 2000 Jan 1;28(1):27-30. (PMID: 10592173)
Biosci Biotechnol Biochem. 2013;77(5):966-70. (PMID: 23649263)
Biochem Biophys Res Commun. 2002 Jan 18;290(2):824-9. (PMID: 11785976)
Environ Sci Technol. 2011 Jul 15;45(14):6117-23. (PMID: 21682287)
J Bacteriol. 2008 Apr;190(8):2997-3005. (PMID: 18263722)

EC 1.1.1.27 (L-Lactate Dehydrogenase)
0 (Malates)
0 (Ketoglutaric Acids)
33X04XA5AT (Lactic Acid)
0 (Succinates)

Date Created: 20230504 Date Completed: 20230508 Latest Revision: 20230511

20250114

PMC10160077

10.1038/s41598-023-34289-3

37142758