OsRbohI Regulates Rice Growth and Development via Jasmonic Acid Signalling.

Publisher: Oxford University Press Country of Publication: Japan NLM ID: 9430925 Publication Model: Print Cited Medium: Internet ISSN: 1471-9053 (Electronic) Linking ISSN: 00320781 NLM ISO Abbreviation: Plant Cell Physiol Subsets: MEDLINE

Publication: Tokyo : Oxford University Press
Original Publication: Kyoto, Japan : Japanese Society of Plant Physiologists,

Plant Proteins*/genetics ; Plant Proteins*/metabolism ; Oryza*/metabolism; Reactive Oxygen Species/metabolism ; Plant Roots/metabolism ; Oxylipins/pharmacology ; Oxylipins/metabolism ; Cyclopentanes/pharmacology ; Cyclopentanes/metabolism ; Signal Transduction ; Growth and Development ; Gene Expression Regulation, Plant

Reactive oxygen species (ROS) are highly reactive molecules, generated by nicotinamide adenine dinucleotide phosphate oxidases encoded by respiratory burst oxidase homologs. The functions of the OsRbohs gene family in rice are diverse and poorly understood. OsRbohI was recently identified as a newly evolved gene in the rice OsRbohs gene family. However, the function of OsRbohI in regulating rice growth is not yet reported. In this study, our results indicate that knockout (KO) OsRbohI mutants showed significantly shorter shoot and primary roots, along with lower ROS content than the control lines, whereas the overexpression (OE) lines displayed contrasting results. Further experiments showed that the abnormal length of the shoot and root is mainly caused by altered cell size. These results indicate that OsRbohI regulates rice shoot and root growth through the ROS signal. More importantly, RNA-seq analysis and jasmonic acid (JA) treatment demonstrated that OsRbohI regulates rice growth via the JA synthesis and signaling pathways. Compared with the control, the results showed that the KO mutants were more sensitive to JA, whereas the OE lines were less sensitive to JA. Collectively, our results reveal a novel pathway in which OsRbohI regulates rice growth and development by affecting their ROS homeostasis through JA synthesis and signaling pathway.
(© The Author(s) 2023. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Grant No. LZ22C130002 Zhejiang Provincial Natural Science Foundation of China; 2021YFF1000400 the National Key R & D Program of China

Keywords: OsRbohI; Growth; H2O2; JA; ROS; Rice

6RI5N05OWW (jasmonic acid)
0 (Plant Proteins)
0 (Reactive Oxygen Species)
0 (Oxylipins)
0 (Cyclopentanes)

Date Created: 20230410 Date Completed: 20230619 Latest Revision: 20230619

20240829

10.1093/pcp/pcad031

37036744