The Investigation of the Chemical Composition and Applicability of Gold Nanoparticles Synthesized with Amygdalus communis (Almond) Leaf Aqueous Extract as Antimicrobial and Anticancer Agents.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, c1995-

Prunus dulcis* ; Metal Nanoparticles*/chemistry ; Anti-Infective Agents*/pharmacology ; Anti-Infective Agents*/chemistry ; Antineoplastic Agents*/pharmacology ; Antineoplastic Agents*/chemistry; Humans ; Gold/pharmacology ; Gold/chemistry ; Caco-2 Cells ; Tandem Mass Spectrometry ; Anti-Bacterial Agents/chemistry ; Plant Extracts/pharmacology ; Plant Extracts/chemistry ; Green Chemistry Technology

The current work's main objective was to determine the chemical composition of Amygdalus communis (AC) leaf extract and examine the antibacterial and cytotoxic properties of biosynthesized gold nanoparticles (AuNPs). The chemical composition of AC leaf extract was determined using LC-ESI/MS/MS to detect compounds that may be responsible for the reducing, stabilizing, and capping steps in the synthesis of nanoparticles and their biological activities. The AC-AuNPs were spherical, with a particle size lower than 100 nm and a face-centered cubic structure. The EDX spectrum confirmed the formation of AuNPs and a negative zeta potential value (-27.7 mV) suggested their physicochemical stability. The in vitro cytotoxic efficacy of the AC-AuNPs against colorectal adenocarcinoma (Caco-2), glioma (U118), and ovarian (Skov-3) cancer cell lines and human dermal fibroblasts (HDFs) was evaluated by MTT assay. CaCo-2 cell proliferation was effectively inhibited by the AC-AuNPs at concentrations between 25 and 100 g mL ^-1 . The AC-AuNPs exerted preeminent antimicrobial activity against Bacillus subtilis with an MIC of 0.02 μg/mL, whilst good activity was shown against Staphylococcus aureus bacteria and Candida albicans yeast with an MIC of 0.12 μg/mL. Ultimately, the results support the high antibacterial and anticancer potential of biosynthesized AuNPs from AC leaf extract.

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JP 07/22 University of Kragujevac

Keywords: Amygdalus communis L.; AuNPs; anticancer; antimicrobial; chemical composition; green nanotechnology; nanomedicine

7440-57-5 (Gold)
0 (Anti-Infective Agents)
0 (Anti-Bacterial Agents)
0 (Antineoplastic Agents)
0 (Plant Extracts)

Date Created: 20230329 Date Completed: 20230330 Latest Revision: 20230331

20240829

PMC10051629

10.3390/molecules28062428

36985400