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Isolation, identification, molecular typing, and drug resistance of Escherichia coli from infected cattle and sheep in Xinjiang, China.

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اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: John Wiley & Sons Ltd Country of Publication: England NLM ID: 101678837 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2053-1095 (Electronic) Linking ISSN: 20531095 NLM ISO Abbreviation: Vet Med Sci Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: [Oxford] : John Wiley & Sons Ltd., [2015]-
    • الموضوع:
      Escherichia coli Infections*/epidemiology ; Escherichia coli Infections*/veterinary ; Escherichia coli Infections*/microbiology ; Cattle Diseases*/epidemiology ; Cattle Diseases*/microbiology ; Sheep Diseases*/epidemiology; Animals ; Cattle ; Sheep ; Escherichia coli ; Phylogeny ; RNA, Ribosomal, 16S ; China/epidemiology ; Multiplex Polymerase Chain Reaction/veterinary ; Molecular Typing/veterinary ; Drug Resistance
    • نبذة مختصرة :
      Background: Escherichia coli infections are common in Xinjiang, a major region of cattle and sheep breeding in China. Therefore, strategies are required to control E. coli. The aim of this study was to investigate the phylogenetic groups, virulence genes, and antibiotic resistance characteristics of E. coli isolates.
      Methods: In this study, 116 tissue samples were collected from the organs of cattle and sheep that were suspected of having E. coli infections between 2015 and 2019. Bacteria in the samples were identified using a biochemical identification system and amplification of 16S rRNA, and the phylogenetic groupings of E. coli isolates were determined by multiplex polymerase chain reactions. In addition, PCR detection and analysis of virulence factors, antibiotic resistance genes, and drug-resistant phenotypes of E. coli isolates were performed.
      Results: A total of 116 pathogenic E. coli strains belonging to seven phylogenetic groups were isolated, with the majority of isolates in groups A and B1. Among the virulence genes, curli-encoding crl had the highest detection rate of 97.4%, followed by hemolysin-encoding hlyE with the detection rate of 94.82%. Antimicrobial susceptibility test results indicated that the isolates had the highest rates of resistance against streptomycin (81.9%).
      Conclusion: These characteristics complicate the prevention and treatment of E. coli-related diseases in Xinjiang.
      (© 2023 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd.)
    • References:
      Annu Rev Microbiol. 2000;54:641-79. (PMID: 11018140)
      Microb Pathog. 2021 Jun;155:104920. (PMID: 33930418)
      Microb Ecol. 2013 Jan;65(1):84-90. (PMID: 22903163)
      Antimicrob Agents Chemother. 2004 Sep;48(9):3332-7. (PMID: 15328093)
      Poult Sci. 2019 Apr 1;98(4):1791-1804. (PMID: 30544256)
      Lancet Infect Dis. 2018 Jan;18(1):37-46. (PMID: 28864030)
      Mol Microbiol. 2006 Jun;60(5):1136-51. (PMID: 16689791)
      Microbiology (Reading). 2016 Apr;162(4):642-650. (PMID: 26822436)
      ScientificWorldJournal. 2014;2014:289024. (PMID: 25105155)
      Sci Total Environ. 2016 Sep 1;563-564:366-76. (PMID: 27139307)
      J Antimicrob Chemother. 2018 Jun 1;73(6):1460-1463. (PMID: 29481657)
      Int Microbiol. 2022 May;25(2):297-307. (PMID: 34705131)
      Vet Med Sci. 2023 May;9(3):1359-1368. (PMID: 36977209)
      Emerg Infect Dis. 2012 Mar;18(3):439-48. (PMID: 22377426)
      J Am Geriatr Soc. 2006 Apr;54(4):680-4. (PMID: 16686882)
      Prev Vet Med. 2004 Dec 15;66(1-4):175-206. (PMID: 15579343)
      PLoS One. 2016 Jan 25;11(1):e0147954. (PMID: 26809117)
      Environ Microbiol Rep. 2013 Feb;5(1):58-65. (PMID: 23757131)
      Cell. 1996 Nov 29;87(5):791-4. (PMID: 8945505)
      Clin Microbiol Rev. 2004 Oct;17(4):840-62, table of contents. (PMID: 15489351)
      J Appl Microbiol. 2003;95(3):428-36. (PMID: 12911689)
      Antimicrob Agents Chemother. 2006 Nov;50(11):3953-5. (PMID: 16954321)
      Vet Microbiol. 2014 Jan 10;168(1):234-9. (PMID: 24309402)
      Appl Environ Microbiol. 2011 Oct;77(20):7121-7. (PMID: 21856840)
      Gut. 2007 May;56(5):669-75. (PMID: 17028128)
      Sci Adv. 2021 Jan 27;7(5):. (PMID: 33571116)
      PLoS Pathog. 2014 May 08;10(5):e1004124. (PMID: 24809621)
      Appl Environ Microbiol. 2016 Oct 14;82(21):6326-6334. (PMID: 27542931)
      Eur J Clin Microbiol Infect Dis. 2009 Aug;28(8):899-908. (PMID: 19238467)
      Int J Environ Res Public Health. 2020 Jan 06;17(1):. (PMID: 31935799)
      J Med Microbiol. 2007 Apr;56(Pt 4):565-567. (PMID: 17374902)
      J Infect Dev Ctries. 2017 Apr 30;11(4):306-313. (PMID: 28459221)
    • Contributed Indexing:
      Keywords: Escherichia coli infections; antibiotic resistance; cattle; multiplex polymerase chain reaction; phylogeny; sheep; virulence factors
    • الرقم المعرف:
      0 (RNA, Ribosomal, 16S)
    • الموضوع:
      Date Created: 20230328 Date Completed: 20230518 Latest Revision: 20230530
    • الموضوع:
      20231215
    • الرقم المعرف:
      PMC10188099
    • الرقم المعرف:
      10.1002/vms3.1101
    • الرقم المعرف:
      36977209