Streptavidin-Saporin: Converting Biotinylated Materials into Targeted Toxins.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101530765 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6651 (Electronic) Linking ISSN: 20726651 NLM ISO Abbreviation: Toxins (Basel) Subsets: MEDLINE

Original Publication: Basel : MDPI

Immunotoxins*/pharmacology; Animals ; Saporins ; Streptavidin ; Ribosome Inactivating Proteins, Type 1 ; Cell Death ; Plant Proteins/pharmacology ; N-Glycosyl Hydrolases

Streptavidin-Saporin can be considered a type of 'secondary' targeted toxin. The scientific community has taken advantage of this conjugate in clever and fruitful ways using many kinds of biotinylated targeting agents to send saporin into a cell selected for elimination. Saporin is a ribosome-inactivating protein that causes inhibition of protein synthesis and cell death when delivered inside a cell. Streptavidin-Saporin, mixed with biotinylated molecules to cell surface markers, results in powerful conjugates that are used both in vitro and in vivo for behavior and disease research. Streptavidin-Saporin harnesses the 'Molecular Surgery' capability of saporin, creating a modular arsenal of targeted toxins used in applications ranging from the screening of potential therapeutics to behavioral studies and animal models. The reagent has become a well-published and validated resource in academia and industry. The ease of use and diverse functionality of Streptavidin-Saporin continues to have a significant impact on the life science industry.

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Keywords: Streptavidin-ZAP; animal models; behavioral studies; biotinylation; cancer research; engraftment; immunolesioning; immunotoxins; molecular surgery; saporin; secondary conjugate; targeted toxin

EC 3.2.2.22 (Saporins)
0 (Immunotoxins)
9013-20-1 (Streptavidin)
0 (Ribosome Inactivating Proteins, Type 1)
0 (Plant Proteins)
EC 3.2.2.- (N-Glycosyl Hydrolases)

Date Created: 20230328 Date Completed: 20230330 Latest Revision: 20230410

20230410

PMC10059012

10.3390/toxins15030181

36977072