Pseudomonas aeruginosa and Bacillus cereus Isolated from Brazilian Cerrado Soil Act as Phosphate-Solubilizing Bacteria.

Publisher: Springer International Country of Publication: United States NLM ID: 7808448 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-0991 (Electronic) Linking ISSN: 03438651 NLM ISO Abbreviation: Curr Microbiol Subsets: MEDLINE

Original Publication: New York, Springer International.

Phosphates* ; Pseudomonas aeruginosa*/genetics; Bacillus cereus/genetics ; Soil/chemistry ; RNA, Ribosomal, 16S/genetics ; Brazil ; Soil Microbiology

The phosphate-solubilizing microorganism is essential for soil quality and plant development and can serve as an alternative to reduce such Brazilian needs for importing phosphate overseas. Here, we isolated and selected bacteria from Brazilian Cerrado soils capable of solubilize phosphate. We obtained 53 bacteria isolates, of which 23 could solubilize phosphate at a pH of 7.0, 17 could solubilize phosphate at a pH of 6.0, and 8 could solubilize at a pH of 5.5. Using 16S rRNA gene sequences, we identified nine bacteria species clustered in four groups: Bacillus sp., Pseudomonas sp., Priestia sp., and Klebsiella sp. Our results revealed that the UFT01 (P. aeruginosa) and UFT42 (B. cereus) isolates exhibited the best phosphate solubilization performance at all tested pH values. We further recorded higher levels of solubilization and phosphate availability six days after the soil inoculation with P. aeruginosa, and enzymatic analysis of the soil samples revealed that the P. aeruginosa-inoculated samples resulted in four-fold higher enzymatic activities when compared to non-inoculated soils. The B. cereus soil inoculation increased β-glucosidase activities and resulted in reduced the activities of arylsulfatase. Altogether, our findings demonstrated that P. aeruginosa and B. cereus isolated from Cerrado soils showed high phosphate solubilization potential.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Backer R, Rokem JS, Ilangumaran G, Lamont J, Praslickova D, Ricci E, Subramanian S, Smith DL (2018) Plant growth-promoting rhizobacteria: context, mechanisms of action, and roadmap to commercialization of biostimulants for sustainable agriculture. Front Plant Sci. https://doi.org/10.3389/fpls.2018.01473. (PMID: 10.3389/fpls.2018.01473304056526206271)
Mogollón JM, Bouwman AF, Beusen AHW, Lassaletta L, van Grinsven HJM, Westhoek H (2021) More efficient phosphorus use can avoid cropland expansion. Nat Food 2(7):509–518. https://doi.org/10.1038/s43016-021-00303-y. (PMID: 10.1038/s43016-021-00303-y)
Gomes L, Simões SJC, Dalla Nora EL, de Sousa-Neto ER, Forti MC, Ometto JPHB (2019) Agricultural expansion in the Brazilian Cerrado: increased soil and nutrient losses and decreased agricultural productivity. Land 8(1):12. (PMID: 10.3390/land8010012)
Hawkins JMB, Vermeiren C, Blackwell MSA, Darch T, Granger SJ, Dunham SJ, Hernandez-Allica J, Smolders E, McGrath S (2022) The effect of soil organic matter on long-term availability of phosphorus in soil: evaluation in a biological P mining experiment. Geoderma 423:115965. https://doi.org/10.1016/j.geoderma.2022.115965. (PMID: 10.1016/j.geoderma.2022.115965)
Fitzpatrick CR, Copeland J, Wang PW, Guttman DS, Kotanen PM, Johnson MTJ (2018) Assembly and ecological function of the root microbiome across angiosperm plant species. Proc Natl Acad Sci 115(6):E1157–E1165. https://doi.org/10.1073/pnas.1717617115. (PMID: 10.1073/pnas.1717617115293584055819437)
Nannipieri P, Trasar-Cepeda C, Dick RP (2018) Soil enzyme activity: a brief history and biochemistry as a basis for appropriate interpretations and meta-analysis. Biol Fert Soils 54(1):11–19. https://doi.org/10.1007/s00374-017-1245-6. (PMID: 10.1007/s00374-017-1245-6)
Wang S, Zhou K, Mori T, Mo J, Zhang W (2019) Effects of phosphorus and nitrogen fertilization on soil arylsulfatase activity and sulfur availability of two tropical plantations in southern China. Forest Ecol Manag 453:117613. https://doi.org/10.1016/j.foreco.2019.117613. (PMID: 10.1016/j.foreco.2019.117613)
Agriculture USDo (2020) Keys to soil taxonomy: updated edition - U.S. Department of Agriculture (USDA). Independently Published, Chicago.
Bortolon L, Gianello C (2010) Simultaneous multielement extraction with the Mehlich-1 solution for Southern Brazilian soils determined by ICP-OES and the effects on the nutrients recommendations to crops. Rev Bras Cienc Solo. https://doi.org/10.1590/S0100-06832010000100013. (PMID: 10.1590/S0100-06832010000100013)
Akinrinlola RJ, Yuen GY, Drijber RA, Adesemoye AO (2018) Evaluation of bacillus strains for plant growth promotion and predictability of efficacy by in vitro physiological traits. Int J Microbiol 2018:5686874. https://doi.org/10.1155/2018/5686874. (PMID: 10.1155/2018/5686874304021056192143)
Walch G, Knapp M, Rainer G, Peintner U (2016) Colony-PCR is a rapid method for DNA amplification of hyphomycetes. J Fungi 2(2):12. (PMID: 10.3390/jof2020012)
Carroll LM, Cheng RA, Wiedmann M, Kovac J (2022) Keeping up with the Bacillus cereus group: taxonomy through the genomics era and beyond. Crit Rev Food Sci Nutr 62(28):7677–7702. https://doi.org/10.1080/10408398.2021.1916735. (PMID: 10.1080/10408398.2021.191673533939559)
Alzohairy A (2011) BioEdit: an important software for molecular biology. GERF Bull Biosci 2:60–61.
Lorenz N, Gardener BBM, Lee NR, Ramsier C, Dick RP (2020) Soil enzyme activities associated with differential outcomes of contrasting approaches to soil fertility management in corn and soybean fields. Appl Ecol Environ Sci 8(6):517–525. https://doi.org/10.12691/aees-8-6-26. (PMID: 10.12691/aees-8-6-26)
Ducousso-Détrez A, Fontaine J, Lounès-Hadj Sahraoui A, Hijri M (2022) Diversity of phosphate chemical forms in soils and their contributions on soil microbial community structure changes. Microorganisms 10(3):609. (PMID: 10.3390/microorganisms10030609353361848950675)
Procópio L, Barreto C (2021) The soil microbiomes of the Brazilian Cerrado. J Soil Sediment 21(6):2327–2342. https://doi.org/10.1007/s11368-021-02936-9. (PMID: 10.1007/s11368-021-02936-9)
Cueva-Yesquén LG, Goulart MC, Attili de Angelis D, Nopper Alves M, Fantinatti-Garboggini F (2021) Multiple plant growth-promotion traits in endophytic bacteria retrieved in the vegetative stage from passionflower. Front Plant Sci. https://doi.org/10.3389/fpls.2020.621740. (PMID: 10.3389/fpls.2020.621740335370517847900)
Vekataramappa R, Mahadev NK, Venkatesh S, Balakrishanan K, Suryan S, Govinahalli Shivashankar N, Seshagiri DS (2022) The isolation, characterization and identification of multifaceted halotolerant bacillus licheniformis and bacillus wudalianchiensis from rhizospheric soils of bangalore. J Microbiol Biotechnol Food Sci 11(6):e3553. https://doi.org/10.55251/jmbfs.3553. (PMID: 10.55251/jmbfs.3553)
Ren H, Qin X, Huang B, Fernández-García V, Lv C (2020) Responses of soil enzyme activities and plant growth in a eucalyptus seedling plantation amended with bacterial fertilizers. Arch Microbiol 202(6):1381–1396. https://doi.org/10.1007/s00203-020-01849-4. (PMID: 10.1007/s00203-020-01849-432179939)
Bhakthavatchalu S, Shivakumar S (2018) The influence of physicochemical parameters on phosphate solubilization and the biocontrol traits of Pseudomonas aeruginosa FP6 in phosphate-deficient conditions. Jordan J Biol Sci 11:215–224.
Chagas L, Chagas A, Carvalho M, Miller L, Orozco Colonia B (2015) Evaluation of the phosphate solubilization potential of Trichoderma strains (Trichoplus JCO) and effects on rice biomass. J Soil Sci Plant Nutr 15:794–804. https://doi.org/10.4067/S0718-95162015005000054. (PMID: 10.4067/S0718-95162015005000054)
Fiuza DAF, Vitorino LC, Souchie EL, Neto MR, Bessa LA, Silva CFd, Trombela NT (2022) Effect of Rhizobacteria inoculation via soil and seeds on Glycine max L. Plants Grown Soils Differ Crop Hist 10(4):691.
Elhaissoufi W, Ghoulam C, Barakat A, Zeroual Y, Bargaz A (2022) Phosphate bacterial solubilization: a key rhizosphere driving force enabling higher P use efficiency and crop productivity. J Adv Res 38:13–28. https://doi.org/10.1016/j.jare.2021.08.014. (PMID: 10.1016/j.jare.2021.08.01435572398)
Hemkemeyer M, Schwalb SA, Heinze S, Joergensen RG, Wichern F (2021) Functions of elements in soil microorganisms. Microbiol Res 252:126832. https://doi.org/10.1016/j.micres.2021.126832. (PMID: 10.1016/j.micres.2021.12683234508963)
Abdelgalil SA, Kaddah MMY, Duab MEA, Abo-Zaid GA (2022) A sustainable and effective bioprocessing approach for improvement of acid phosphatase production and rock phosphate solubilization by Bacillus haynesii strain ACP1. Sci Rep UK 12(1):8926. https://doi.org/10.1038/s41598-022-11448-6. (PMID: 10.1038/s41598-022-11448-6)
Stege PW, Messina GA, Bianchi G, Olsina RA, Raba J (2010) Determination of beta-glucosidase activity in soils with a bioanalytical sensor modified with multiwalled carbon nanotubes. Anal Bioanal Chem 397(3):1347–1353. https://doi.org/10.1007/s00216-010-3634-7. (PMID: 10.1007/s00216-010-3634-720349226)
Li W, Wu M, Liu M, Jiang C, Chen X, Kuzyakov Y, Rinklebe J, Li Z (2018) Responses of soil enzyme activities and microbial community composition to moisture regimes in paddy soils under long-term fertilization practices. Pedosphere 28(2):323–331. https://doi.org/10.1016/S1002-0160(18)60010-4. (PMID: 10.1016/S1002-0160(18)60010-4)
Chen H, Liu J, Li D, Xiao K, Wang K (2019) Controls on soil arylsulfatase activity at a regional scale. Eur J Soil Biol 90:9–14. https://doi.org/10.1016/j.ejsobi.2018.11.001. (PMID: 10.1016/j.ejsobi.2018.11.001)

Finnance Code 01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; 313455/2019-8 Conselho Nacional de Desenvolvimento Científico e Tecnológico

0 (Phosphates)
0 (Soil)
0 (RNA, Ribosomal, 16S)

Date Created: 20230323 Date Completed: 20230327 Latest Revision: 20230327

20240513

10.1007/s00284-023-03260-w

36952131