Antioxidant Activity of Polysaccharides from the Edible Mushroom Pleurotus eryngii .

Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, c1995-

Agaricales*/metabolism ; Pleurotus*/chemistry; Antioxidants/chemistry ; Superoxides/metabolism ; Nitric Oxide/metabolism ; Hydrogen Peroxide/metabolism ; Polysaccharides/chemistry ; Hydroxyl Radical/metabolism

In this study the antioxidant and neuroprotective activity of an enriched polysaccharide fraction (EPF) obtained from the fruiting body of cultivated P. eryngii was evaluated. Proximate composition (moisture, proteins, fat, carbohydrates and ash) was determined using the AOAC procedures. The EPF was extracted by using, in sequence, hot water and alkaline extractions followed by deproteinization and precipitation with cold ethanol. Total α- and β-glucans were quantified using the Megazyme International Kit. The results showed that this procedure allows a high yield of polysaccharides with a higher content of (1-3; 1-6)-β-D-glucans. The antioxidant activity of EPF was detected from the total reducing power, DPPH, superoxide, hydroxyl and nitric oxide radical scavenging activities. The EPF was found to scavenge DPPH, superoxide, hydroxyl and nitric oxide radicals with a IC50 values of 0.52 ± 0.02, 1.15 ± 0.09, 0.89 ± 0.04 and 2.83 ± 0.16 mg/mL, respectively. As assessed by the MTT assay, the EPF was biocompatible for DI-TNC1 cells in the range of 0.006-1 mg/mL and, at concentrations ranging from 0.05 to 0.2 mg/mL, significantly counteracted H 2 O 2 -induced reactive oxygen species production. This study demonstrated that polysaccharides extracted from P. eryngii might be used as functional food to potentiate the antioxidant defenses and to reduce oxidative stress.

Anal Biochem. 1973 Aug;54(2):484-9. (PMID: 4269305)
Carbohydr Polym. 2021 Jul 15;264:117991. (PMID: 33910729)
J Neurochem. 2018 Feb;144(3):271-284. (PMID: 29210076)
Autoimmune Dis. 2011 Feb 24;2010:249842. (PMID: 21461338)
Recent Pat Food Nutr Agric. 2019;10(1):3-19. (PMID: 29866029)
Food Chem. 2017 Feb 1;216:45-51. (PMID: 27596390)
Ann Transl Med. 2014 Feb;2(2):17. (PMID: 25332993)
Foods. 2016 Nov 29;5(4):. (PMID: 28231175)
Arch Biochem Biophys. 1984 Nov 15;235(1):116-26. (PMID: 6093705)
Curr Opin Biotechnol. 2014 Apr;26:162-73. (PMID: 24518400)
Int J Med Mushrooms. 2022;24(9):15-24. (PMID: 36004706)
Molecules. 2022 Nov 23;27(23):. (PMID: 36500238)
Int J Biol Macromol. 2019 Feb 15;123:945-956. (PMID: 30447375)
Int J Med Mushrooms. 2015;17(10):987-96. (PMID: 26756190)
Front Nutr. 2023 Jan 25;10:1118923. (PMID: 36761225)
Anal Biochem. 1976 May 7;72:248-54. (PMID: 942051)
J Mol Neurosci. 2021 Mar;71(3):506-514. (PMID: 32767188)
J AOAC Int. 2016 Mar-Apr;99(2):364-73. (PMID: 26957216)
Carbohydr Polym. 2014 Dec 19;114:297-305. (PMID: 25263894)
Molecules. 2015 Oct 27;20(10):19489-525. (PMID: 26516828)
Oxid Med Cell Longev. 2016;2016:1245049. (PMID: 27478531)
Int J Food Sci Nutr. 2017 May;68(3):287-297. (PMID: 27760471)
Int J Biol Macromol. 2010 Aug 1;47(2):116-9. (PMID: 20580645)
Antioxidants (Basel). 2020 Jul 22;9(8):. (PMID: 32707949)
RSC Adv. 2020 Mar 24;10(20):11918-11928. (PMID: 35694324)
Int J Biochem Cell Biol. 2007;39(1):44-84. (PMID: 16978905)
Carbohydr Polym. 2020 Feb 1;229:115550. (PMID: 31826512)
Food Chem. 2016 Apr 15;197(Pt A):240-9. (PMID: 26616946)
Int J Mol Sci. 2022 May 25;23(11):. (PMID: 35682615)
Curr Med Chem. 2009;16(12):1543-60. (PMID: 19355906)
Curr Nutr Rep. 2019 Jun;8(2):53-65. (PMID: 30949921)
Front Immunol. 2022 May 02;13:859923. (PMID: 35585984)
Food Funct. 2018 Jul 17;9(7):3764-3775. (PMID: 29897364)
Appl Microbiol Biotechnol. 2002 Nov;60(3):258-74. (PMID: 12436306)
Molecules. 2020 Sep 24;25(19):. (PMID: 32987656)
Int J Biol Macromol. 2016 Nov;92:30-36. (PMID: 27392770)
Int J Biol Macromol. 2020 May 1;150:1342-1347. (PMID: 31759001)
Neural Regen Res. 2022 Sep;17(9):1907-1912. (PMID: 35142666)
Food Chem. 2013 Jun 1;138(2-3):1557-63. (PMID: 23411281)
Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6467-71. (PMID: 1378628)
Carbohydr Polym. 2015 Mar 6;117:753-761. (PMID: 25498697)
Int J Med Mushrooms. 2018;20(8):717-726. (PMID: 30317948)
Carbohydr Res. 2016 Nov 3;434:72-76. (PMID: 27599128)
Inflammopharmacology. 2021 Apr;29(2):561-571. (PMID: 33196947)
Neuroscience. 2018 Apr 15;376:48-71. (PMID: 29462705)
Int J Med Mushrooms. 2020;22(4):325-333. (PMID: 32558497)
Braz J Microbiol. 2014 Mar 10;44(4):1081-8. (PMID: 24688496)
Int J Med Mushrooms. 2011;13(5):401-26. (PMID: 22324407)
Int J Biol Macromol. 2014 Apr;65:28-32. (PMID: 24418337)
Compr Rev Food Sci Food Saf. 2016 Mar;15(2):237-250. (PMID: 33371599)
Evid Based Complement Alternat Med. 2005 Sep;2(3):285-99. (PMID: 16136207)
Int J Med Mushrooms. 2020;22(12):1171-1181. (PMID: 33463934)
Food Funct. 2017 Sep 20;8(9):3288-3296. (PMID: 28832062)
Molecules. 2022 Oct 21;27(20):. (PMID: 36296732)

CUP G29J21005620006 PSR 2014-2020 Regione Basilicata, Misura 16, Sottomisura 16.2-Sostegno a progetti pilota e allo sviluppo di nuovi prodotti, pratiche, processi e tecnologie. Innovazione tecnologica e di processo per la produzione in continuo di funghi coltivati in biologi

Keywords: Pleurotus eryngii; antioxidant activity; mushrooms; neuroprotection; polysaccharides; β-glucan

0 (Antioxidants)
11062-77-4 (Superoxides)
31C4KY9ESH (Nitric Oxide)
BBX060AN9V (Hydrogen Peroxide)
0 (Polysaccharides)
3352-57-6 (Hydroxyl Radical)

Pleurotus eryngii

Date Created: 20230311 Date Completed: 20230314 Latest Revision: 20230314

20240628

PMC10005153

10.3390/molecules28052176

36903422