MitoSNARE Assembly and Disassembly Factors Regulate Basal Autophagy and Aging in C. elegans .

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Autophagy* ; Caenorhabditis elegans*/physiology ; SNARE Proteins*/physiology ; Aging*; Animals ; Endocytosis ; Membrane Fusion

SNARE proteins reside between opposing membranes and facilitate vesicle fusion, a physiological process ubiquitously required for secretion, endocytosis and autophagy. With age, neurosecretory SNARE activity drops and is pertinent to age-associated neurological disorders. Despite the importance of SNARE complex assembly and disassembly in membrane fusion, their diverse localization hinders the complete understanding of their function. Here, we revealed a subset of SNARE proteins, the syntaxin SYX-17, the synaptobrevins VAMP-7, SNB-6 and the tethering factor USO-1, to be either localized or in close proximity to mitochondria, in vivo. We term them mitoSNAREs and show that animals deficient in mitoSNAREs exhibit increased mitochondria mass and accumulation of autophagosomes. The SNARE disassembly factor NSF-1 seems to be required for the effects of mitoSNARE depletion. Moreover, we find mitoSNAREs to be indispensable for normal aging in both neuronal and non-neuronal tissues. Overall, we uncover a previously unrecognized subset of SNAREs that localize to mitochondria and propose a role of mitoSNARE assembly and disassembly factors in basal autophagy regulation and aging.

EMBO J. 2014 Oct 1;33(19):2142-56. (PMID: 25107473)
Nat Rev Mol Cell Biol. 2021 Nov;22(11):733-750. (PMID: 34302147)
Nucleic Acids Res. 2022 Apr 30;:. (PMID: 35489069)
Neuron. 2020 Jul 8;107(1):22-37. (PMID: 32559416)
Am J Physiol Cell Physiol. 2011 Apr;300(4):C723-42. (PMID: 21209361)
Dev Cell. 2015 Feb 9;32(3):304-17. (PMID: 25619926)
Mol Biol Evol. 2008 Sep;25(9):2055-68. (PMID: 18621745)
Curr Biol. 2018 Apr 23;28(8):R397-R401. (PMID: 29689222)
Angew Chem Int Ed Engl. 2014 Nov 17;53(47):12696-717. (PMID: 25339369)
EMBO J. 2014 Feb 18;33(4):282-95. (PMID: 24446486)
Mol Biol Cell. 2007 Sep;18(9):3463-71. (PMID: 17596510)
Trends Biochem Sci. 2013 Feb;38(2):57-63. (PMID: 23306003)
Genetics. 2015 Feb;199(2):511-22. (PMID: 25519897)
Mol Syst Biol. 2011 Oct 11;7:539. (PMID: 21988835)
J Neurochem. 2010 Jun;113(6):1577-88. (PMID: 20374424)
J Neurosci Res. 2009 Jun;87(8):1760-72. (PMID: 19185015)
Methods Mol Biol. 2019;1880:561-586. (PMID: 30610723)
Autophagy. 2014 Mar;10(3):431-41. (PMID: 24394643)
WormBook. 2014 Apr 25;:1-47. (PMID: 24778088)
Cell Metab. 2021 Dec 7;33(12):2464-2483.e18. (PMID: 34800366)
Nat Commun. 2022 Jan 20;13(1):424. (PMID: 35058456)
Nat Rev Mol Cell Biol. 2018 Jun;19(6):349-364. (PMID: 29618831)
Cells. 2021 Apr 23;10(5):. (PMID: 33922505)
J Exp Clin Cancer Res. 2021 Jan 14;40(1):31. (PMID: 33446221)
FEBS Lett. 2021 Apr;595(8):1107-1131. (PMID: 33314127)
Pharmacol Res. 2021 Mar;165:105469. (PMID: 33524541)
Cell. 2011 Jul 22;146(2):303-17. (PMID: 21784250)
Autophagy. 2021 Oct;17(10):2680-2688. (PMID: 32924745)
Genome Biol. 2001;2(1):RESEARCH0002. (PMID: 11178279)
Dan Med Bull. 1985 Feb;32 Suppl 1:40-3. (PMID: 3987356)
Bioinformation. 2018 Jul 31;14(7):361-368. (PMID: 30262973)
Nucleic Acids Res. 2021 Jul 2;49(W1):W293-W296. (PMID: 33885785)
Cell Metab. 2015 Sep 1;22(3):499-507. (PMID: 26331606)
Science. 2020 Mar 20;367(6484):1366-1371. (PMID: 32193326)
Nature. 2013 Mar 21;495(7441):389-93. (PMID: 23455425)
Methods Mol Biol. 2013;940:77-86. (PMID: 23104335)
Genetics. 1974 May;77(1):71-94. (PMID: 4366476)
Cell. 2004 Jan 23;116(2):153-66. (PMID: 14744428)
Dev Cell. 2019 Apr 8;49(1):130-144.e6. (PMID: 30827897)
Cell. 2011 Jul 22;146(2):290-302. (PMID: 21784249)
PLoS Genet. 2018 Jul 19;14(7):e1007417. (PMID: 30024879)
Semin Cell Dev Biol. 2016 Dec;60:97-104. (PMID: 27422330)
Curr Biol. 2011 Sep 27;21(18):R697-9. (PMID: 21959158)
Cell. 2012 Dec 7;151(6):1256-69. (PMID: 23217709)
Nat Rev Mol Cell Biol. 2016 Aug;17(8):465-79. (PMID: 27301672)
Sci Adv. 2022 Aug 5;8(31):eabo0412. (PMID: 35921421)
J Cell Sci. 2009 Jun 15;122(Pt 12):2003-13. (PMID: 19454479)
J Cell Sci. 2012 Apr 15;125(Pt 8):1896-909. (PMID: 22328511)
Sci Adv. 2019 Apr 10;5(4):eaau8164. (PMID: 30989110)
J Cell Biol. 2016 Aug 1;214(3):275-91. (PMID: 27458136)
Front Mol Neurosci. 2021 Aug 06;14:733138. (PMID: 34421538)
Front Mol Neurosci. 2017 Mar 21;10:66. (PMID: 28377692)
Nature. 1993 Mar 25;362(6418):318-24. (PMID: 8455717)
Cell Biol Int. 2009 Sep;33(9):934-40. (PMID: 19524684)
Aging (Albany NY). 2015 Jun;7(6):419-34. (PMID: 26142908)
EMBO J. 2018 Nov 2;37(21):. (PMID: 30237312)
J Biol Chem. 2004 Jan 16;279(3):2299-306. (PMID: 14573619)
Biochem Biophys Res Commun. 2005 Dec 16;338(2):1268-74. (PMID: 16256943)
J Cell Biol. 2018 Aug 6;217(8):2633-2645. (PMID: 29789439)
Mol Biol Cell. 1998 Jul;9(7):1649-60. (PMID: 9658161)
Proc Natl Acad Sci U S A. 2018 Dec 11;115(50):12751-12756. (PMID: 30482862)
Annu Rev Biochem. 2017 Jun 20;86:685-714. (PMID: 28301740)
J Cell Sci. 2021 Nov 15;134(22):. (PMID: 34704591)
Neurobiol Dis. 2018 Jun;114:31-44. (PMID: 29496544)
J Cell Biol. 2018 Apr 2;217(4):1369-1382. (PMID: 29382700)
Proc Natl Acad Sci U S A. 1999 Jun 22;96(13):7394-7. (PMID: 10377425)
Eur J Biochem. 1996 Nov 1;241(3):779-86. (PMID: 8944766)
Traffic. 2015 Feb;16(2):148-71. (PMID: 25406594)

Keywords: NSF-1; SNAREs; SYX-17; aging; autophagy; mitochondria

0 (SNARE Proteins)

Date Created: 20230225 Date Completed: 20230228 Latest Revision: 20230301

20240829

PMC9964399

10.3390/ijms24044230

36835643