Characterization of RING-type ubiquitin SINA E3 ligases and their responsive expression to salt and osmotic stresses in Brassica napus.

Publisher: Springer Country of Publication: Germany NLM ID: 9880970 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-203X (Electronic) Linking ISSN: 07217714 NLM ISO Abbreviation: Plant Cell Rep Subsets: MEDLINE

Original Publication: Berlin ; New York : Springer, 1981-

Arabidopsis*/genetics ; Brassica napus*/genetics ; Brassica napus*/metabolism; Ubiquitin-Protein Ligases/genetics ; Ubiquitin-Protein Ligases/metabolism ; Osmotic Pressure ; Ubiquitin/genetics ; Ubiquitin/metabolism ; Phylogeny ; Plant Breeding ; Sodium Chloride/pharmacology ; Sodium Chloride/metabolism ; Stress, Physiological/genetics ; Gene Expression Regulation, Plant ; Plant Proteins/metabolism

SINA (Seven in absentia) proteins in the subtype of E3 ubiquitin ligase family play a crucial role in plant growth and development. However, their functions in response to salt and osmotic stresses in oil crops are still largely unknown. In this study, a total number of 23 BnaSINAs were identified in the rapeseed genome. Chromosome location and collinear relationship analyses revealed that they were unevenly distributed on 13 chromosomes, and have gone through 22 segmental duplication events under purifying selection. Phylogenetic and gene structural analyses indicated that they belonged to five main groups, and those in the same subgroup showed similar gene structure. All BnaSINAs were predicted to form homo- or heterodimers. Except BnaSINA7, BnaSINA11, BnaSINA17 and BnaSINA18, which lacked the N-terminal RING finger, all BnaSINAs contained a conserved C-terminal SINA domain, a typical structural feature of the RING-type E3 ligase family. Transcriptional expression analyses demonstrated that most BnaSINAs were ubiquitously expressed in roots, stems, leaves, flowers, pods and seeds, and all were responsive to salt and osmotic stresses. Further, yeast two-hybrid and Arabidopsis mutant complementation analyses demonstrated that BnaSINA4 interacted with BnaSINA17 to form heterodimer, and expression of BnaSINA17 in the Arabidopsis sina2 mutant restored its growth resistance to salt and osmotic stresses. Our findings provide an important genetic foundation for the functional elucidation of BnaSINAs and a novel gene resource for the breeding of new oil crop cultivars with improved abiotic stress resistance.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

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ZR2019PC015 Natural Science Foundation of Shandong Province; ZR2020QC115 Natural Science Foundation of Shandong Province; 31901572 National Natural Science Foundation of China; 31870576 National Natural Science Foundation of China; 32071733 National Natural Science Foundation of China; 31901987 National Natural Science Foundation of China; 31100218 National Natural Science Foundation of China; 2018YFD01000500 National Key Research and Development Program of China; 2019YFD01000500 National Key Research and Development Program of China; 2021XDRHXMPT09 The Cooperation Project of University and Local Enterprise in Yantai of Shandong Province; SDAIT-02-05 The Modern Agricultural Industry Technology System Innovation Team of Shandong Province of China

Keywords: Brassica napus; E3 ubiquitin ligase; Osmotic stress; SINA; Salt stress

EC 2.3.2.27 (Ubiquitin-Protein Ligases)
0 (Ubiquitin)
451W47IQ8X (Sodium Chloride)
0 (Plant Proteins)

Date Created: 20230214 Date Completed: 20230508 Latest Revision: 20230508

20230508

10.1007/s00299-023-02996-w

36788135