نبذة مختصرة : Euryales Semen was a traditional Chinese medicine, which has been commonly used to treat spermatorrhea, enuresis, and frequent urination. Flavonoids were a critical ingredient in determining the function and quality of Euryales Semen. At present, no effective method has been established for the qualitative of Euryales Semen flavonoids. In this study, an ultra-high-performance liquid chromatography-quadrupole-time of flight-mass spectrometry method was established for flavonoids. By comparison with standard or literature data, 32 flavonoid compounds have been identified in Euryales Semen. Based on the qualitative results, an ultra-high-performance liquid chromatography-triple quadrupole tandem mass spectroscopy method was developed for the main components, and the linearity, the limit of detection, limit of quantification, repeatability, precision, stability, and recovery of the method were verified. The principal component analysis and the hierarchical clustering heatmaps analysis showed that the 30 batches of samples were distinctly separated into the North Gordon Euryale and South Gordon Euryale, and the measured contents of the six flavonoids in North Gordon Euryale were more abundant than in South Gordon Euryale, especially isoquercitrin, hesperetin, and quercetin. It provided a scientific basis for the quality control of Euryales Semen and a theoretical basis for the rational utilization of Euryales Semen resources.
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References: Huang Z, Bao Ke, Jing Z, Wang Q, Duan H, Zhu Y, Zhang S, Wu Q. Small Auxin Up RNAs influence the distribution of indole-3-acetic acid and play a potential role in increasing seed size in Euryale ferox Salisb. BMC Plant Biol. 2020;20:311.
Bao Ke, Jing Z, Wang Q, Huang Z, Han D, Dai S, Liu C, Wu Q, Xu F. Quality analysis of Euryales Semen from different origins and varieties based on untargeted metabolomics. J Chromatogr B. 2022;1191:123114.
Hostetler GL, Ralston RA, Schwartz SJ. Flavones: Food Sources, Bioavailability, Metabolism, and Bioactivity. Adv Nutr. 2017;8:423-35.
Oshima N, Maruyama T, Hanawa T, Goda Y. Two flavone C-glycosides as quality control markers for the manufacturing process of ephedrine alkaloids-free Ephedra Herb extract (EFE) as a crude drug preparation. J Nat Med. 2018;72:73-9.
Boyle SP, Doolan PJ, Andrews CE, Reid RG. Evaluation of quality control strategies in Scutellaria herbal medicines. J Pharm Biomed Anal. 2011;54:951-7.
Xu W, Huang M, Li H, Chen X, Zhang Y, Liu J, Xu W, Chu K, Chen L. Chemical profiling and quantification of Gua-Lou-Gui-Zhi decoction by high performance liquid chromatography/quadrupole-time-of-flight mass spectrometry and ultra-performance liquid chromatography/triple quadrupole mass spectrometry. J Chromatogr B. 2015;986-987:69-84.
Kind T, Tsugawa H, Arita M, Fiehn O. Identification of small molecules using accurate mass MS/MS search. Mass Spectrom Rev. 2018;37:513-32.
Ma TX, Zhang L, Xu L, Ye YH, Huang T, Zhou QM, Liu HL. Mitigation of isoquercitrin on β-lactoglobulin glycation: Insight into the mechanisms by mass spectrometry and interaction analysis. Int J Biol Macromol. 2020;155:1133-41.
Borriello M, Iannuzzi C, Sirangelo I. Pinocembrin Protects from AGE-Induced Cytotoxicity and Inhibits Non-Enzymatic Glycation in Human Insulin. Cells 2019;8:385.
Zhuang M, Qiu H, Wang Y, Rao L. Islet protection and amelioration of type 2 diabetes mellitus by treatment with quercetin from the flowers of Edgeworthia gardneri. Drug Des Devel Ther. 2018;12:955-66.
Yu KY, Gao W, Li SZ, Wu W, Li P, Dou LL, Wang YZ, Liu EH. Qualitative and quantitative analysis of chemical constituents in Ardisiae Japonicae Herba. J Sep Sci. 2017;40(22):4347-56.
Qu C, Wen J-H, Li P, Gao W, Yang H. Target profiling of flavonol glycosides in the extract of Ginkgo biloba leaf and their pharmacokinetics in rat plasma by ultra-high-performance liquid chromatography with tandem mass spectrometry. J Sep Sci. 2022;45:728-38.
Ahmed D, Kumar V, Verma A, Shankar Shukla G, Sharma M. Antidiabetic, antioxidant, antihyperlipidemic effect of extract of Euryale ferox salisb. with enhanced histopathology of pancreas, liver and kidney in streptozotocin induced diabetic rats. Springerplus 2015;4:315.
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