Glial cells expressing visual cycle genes are vital for photoreceptor survival in the zebrafish pineal gland.

Publisher: Wiley Country of Publication: England NLM ID: 8504412 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1600-079X (Electronic) Linking ISSN: 07423098 NLM ISO Abbreviation: J Pineal Res Subsets: MEDLINE

Publication: : Oxford : Wiley
Original Publication: New York : Liss, c1984-

Neuroglia*/cytology ; Neuroglia*/metabolism ; Photoreceptor Cells, Vertebrate*/metabolism ; Photoreceptor Cells, Vertebrate*/physiology ; Pineal Gland*/cytology ; Pineal Gland*/metabolism ; Visual Perception*/genetics ; Visual Perception*/physiology; Animals ; Extracellular Vesicles/genetics ; Extracellular Vesicles/metabolism ; Gene Expression ; Melatonin ; Meninges/cytology ; Meninges/physiology ; Photoreceptor Cells/cytology ; Photoreceptor Cells/metabolism ; Rhodopsin/metabolism ; Tetraspanin 30/metabolism ; Zebrafish/genetics ; Zebrafish/metabolism

Photoreceptors in the vertebrate eye are dependent on the retinal pigmented epithelium for a variety of functions including retinal re-isomerization and waste disposal. The light-sensitive pineal gland of fish, birds, and amphibians is evolutionarily related to the eye but lacks a pigmented epithelium. Thus, it is unclear how these functions are performed. Here, we ask whether a subpopulation of zebrafish pineal cells, which express glial markers and visual cycle genes, is involved in maintaining photoreceptors. Selective ablation of these cells leads to a loss of pineal photoreceptors. Moreover, these cells internalize exorhodopsin that is secreted by pineal rod-like photoreceptors, and in turn release CD63-positive extracellular vesicles (EVs) that are taken up by pdgfrb-positive phagocytic cells in the forebrain meninges. These results identify a subpopulation of glial cells that is critical for pineal photoreceptor survival and indicate the existence of cells in the forebrain meninges that receive EVs released by these pineal cells and potentially function in waste disposal.
(© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)

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6276/17 Israel Science Foundation; NRF2017-NRF-ISF002-2676 National Research Foundation of Singapore; RG34/20 Singapore Ministry of Education

Keywords: clearance; extracellular vesicles; glia; meninges; rhodopsin; visual cycle

0 (exorhodopsin)
JL5DK93RCL (Melatonin)
9009-81-8 (Rhodopsin)
0 (Tetraspanin 30)
0 (exorh protein, zebrafish)

Date Created: 20230124 Date Completed: 20231026 Latest Revision: 20231026

20231215

10.1111/jpi.12854

36692235