Validation of N-Light™ Salmonella Risk Test Kit for Detection of Salmonella spp. on Environmental Surfaces: AOAC Performance Tested MethodSM 072204.

Publisher: Oxford University Press Country of Publication: England NLM ID: 9215446 Publication Model: Print Cited Medium: Internet ISSN: 1944-7922 (Electronic) Linking ISSN: 10603271 NLM ISO Abbreviation: J AOAC Int Subsets: MEDLINE

Publication: 2020- : Oxford : Oxford University Press
Original Publication: Arlington, VA : AOAC International, c1992-

Polystyrenes* ; Food Microbiology*; Salmonella ; Stainless Steel

Background: The NEMIS N-Light™ Salmonella Risk method uses chemiluminescence designed for the qualitative detection of Salmonella spp. from environmental surface samples.
Objective: To validate the N-Light Salmonella Risk assay using independent and method developer validation studies according to the AOAC Performance Tested MethodsSM (PTM) program for the detection of Salmonella spp. on stainless-steel, polystyrene, and ceramic environmental surfaces.
Method: The N-Light Salmonella Risk assay was evaluated in a matrix study in comparison to the ISO 6579-1:2017 method ("Microbiology of the Food Chain-Horizontal Method for the Detection, Enumeration, and Serotyping of Salmonella-Part 1: Detection of Salmonella spp.") using an unpaired study design. Additional PTM studies performed were inclusivity/exclusivity, robustness, product consistency, and stability.
Results: The N-Light Salmonella Risk assay demonstrated a specific detection of all Salmonella strains tested. In the matrix study, the N-Light Salmonella Risk assay showed no significant differences between presumptive and confirmed results or between candidate and reference method results on the three surfaces evaluated. Data for additional PTM studies met acceptance criteria requirements.
Conclusions: The NEMIS Technologies N-Light Salmonella Risk assay is an effective method for the qualitative detection of Salmonella on stainless-steel, polystyrene, and ceramic environmental surfaces.
Highlights: The NEMIS Technologies N-Light Salmonella Risk assay, which is the first chemiluminescence-based detection system that uses a novel, patented dioxetane compound, allowing for easy and rapid detection of Salmonella.
(© The Author(s) 2023. Published by Oxford University Press on behalf of AOAC INTERNATIONAL.)

J AOAC Int. 2011 Jan-Feb;94(1):335-47. (PMID: 21391512)
Int J Food Microbiol. 2019 Jan 2;288:3-12. (PMID: 29803313)

0 (Polystyrenes)
12597-68-1 (Stainless Steel)

Date Created: 20230120 Date Completed: 20230718 Latest Revision: 20230719

20250114

PMC10350619

10.1093/jaoacint/qsad017

36661307