Spinal Cord Injury Causes Marked Tissue Rearrangement in the Urethra-Experimental Study in the Rat.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Urinary Bladder, Overactive*/etiology ; Spinal Cord Injuries*/complications ; Urinary Bladder, Neurogenic*/complications; Rats ; Female ; Animals ; Urethra ; Urinary Bladder/innervation ; Muscle, Smooth

Traumatic spinal cord injury (SCI) results in the time-dependent development of urinary impairment due to neurogenic detrusor overactivity (NDO) and detrusor-sphincter-dyssynergia (DSD). This is known to be accompanied by massive changes in the bladder wall. It is presently less clear if the urethra wall also undergoes remodelling. To investigate this issue, female rats were submitted to complete spinal transection at the T8/T9 level and left to recover for 1 week and 4 weeks. To confirm the presence of SCI-induced NDO, bladder function was assessed by cystometry under urethane anesthesia before euthanasia. Spinal intact animals were used as controls. Urethras were collected and processed for further analysis. Following thoracic SCI, time-dependent changes in the urethra wall were observed. Histological assessment revealed marked urethral epithelium reorganization in response to SCI, as evidenced by an increase in epithelial thickness. At the muscular layer, SCI resulted in strong atrophy of the smooth muscle present in the urethral sphincter. Innervation was also affected, as evidenced by a pronounced decrease in the expression of markers of general innervation, particularly those present in sensory and sympathetic nerve fibres. The present data show an evident impact of SCI on the urethra, with significant histological rearrangement, accompanied by sensory and sympathetic denervation. It is likely that these changes will affect urethral function and contribute to SCI-induced urinary dysfunction, and they deserve further investigation.

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Prémio Melo e Castro - 2016 Santa Casa da Misericódia de Lisboa; UI/BD/151547/2021 Fundação para a Ciência e Tecnologia, Portugal - Ana Ferreira; SFRH/BD/135868/2018 Fundação para a Ciência e Tecnologia, Portugal - Sílvia Chambel

Keywords: epithelium; micturition; nerve fibres; spinal cord injury; urethra; urethral sphincter

Date Created: 20221223 Date Completed: 20221226 Latest Revision: 20221226

20221226

PMC9783636

10.3390/ijms232415951

36555592