Effects of Ozone Therapy on Chronic Arsenic Poisoning in Rats.

Publisher: Humana Press Country of Publication: United States NLM ID: 7911509 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-0720 (Electronic) Linking ISSN: 01634984 NLM ISO Abbreviation: Biol Trace Elem Res Subsets: MEDLINE

Original Publication: [London, Clifton, N. J.] Humana Press.

Ozone*/pharmacology ; Arsenic*/toxicity ; Arsenic Poisoning* ; Drinking Water*; Rats ; Male ; Animals ; Rats, Wistar ; Tumor Suppressor Protein p53

Arsenic (As) is a toxic metalloid that affects many organs through drinking water. This study aims to examine the efficacy of ozone therapy on chronic arsenic toxicity. Twenty-four male Wistar albino rats were housed in individual cages and grouped as control, As, O 3 , and As + O 3. As was applied by adding 5 mg/kg/day in drinking water for 60 days. Ozone therapy was applied at 0.5 mg/kg/day (i.p.) O 3 in the last 5 days of the experimental period. Tissues were harvested and analyzed for histopathological injury and apoptotic markers. There was no significant difference between the As + O 3 and O 3 groups (p = 0.186 and p = 0.599) for light microscopic criteria: inflammatory cell infiltration and hydropic degeneration in liver tissue.In TUNEL assessments, similar outcomes were obtained in the control and As + O 3 groups. A statistically significant increase was observed in p53 and Caspase 3 (Casp-3) expression levels in the As group compared to the O 3 and As + O 3 groups. There was no significant difference between the As + O 3 and O 3 groups on peritubular hemorrhage and desquamation parameters in kidneys (p = 0.147 and p = 0.094). The KIM-1 expression level was significantly increased in the As group compared to the As + O 3 group (p = 0.01), and the Casp-3 expression level was not significantly changed in the O 3 group compared to the As + O 3 group (p = 0.59). In conclusion, it is determined that ozone therapy has ameliorative effects on the microscopic injury of liver and kidney tissues. In addition to microscopic improvement, KIM-1 gene expression levels were ameliorated in the kidneys. The apoptotic cell counts and the Casp-3 and p53 gene expression levels were decreased by O 3 administration. Thus, ozone therapy can be a treatment choice for As toxicity.
(© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

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Keywords: Arsenic poisoning; Experimental; KIM-1; Liver injury; Ozone; Renal injury

66H7ZZK23N (Ozone)
N712M78A8G (Arsenic)
0 (Drinking Water)
0 (Tumor Suppressor Protein p53)

Date Created: 20221127 Date Completed: 20230621 Latest Revision: 20230621

20230621

10.1007/s12011-022-03486-y

36437433