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Identification of noninvasive diagnostic biomarkers for ectopic pregnancy using data-independent acquisition (DIA)proteomics: a pilot study.

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اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: London : Nature Publishing Group, copyright 2011-
    • الموضوع:
      Proteomics* ; Pregnancy, Ectopic*/diagnostic imaging; Pregnancy ; Female ; Humans ; Pilot Projects ; Chorionic Gonadotropin, beta Subunit, Human ; Biomarkers ; Glutathione Transferase
    • نبذة مختصرة :
      At present, the diagnosis of ectopic pregnancy mainly depends on transvaginal ultrasound and β-hCG. However, these methods may delay diagnosis and treatment time. Therefore, we aimed to screen for serological molecular markers for the early diagnosis of ectopic pregnancy (EP).Using data-independent acquisition (DIA)proteomics, the differential proteins in serum were selected between the intrauterine pregnancy (IP) and EP groups. Then, the expression levels of these differential proteins were measured by enzyme-linked immunosorbent assay. The diagnostic value of the serum biomarkers was evaluated by receiver operating characteristic curve analysis.GSTO1, ECM-1 and β-hCG showed significant differences between the EP and IP groups (P < 0.05). The combination of GSTO1/ECM-1/β-hCG had an area under the curve of 0.93 (95% CI 0.88-0.99), a sensitivity of 88.89% (95% CI 73.94-96.89) and a specificity of 86.11% (95% CI 70.50-95.33) with a likelihood ratio of 6.40.The combination of GSTO1/ECM-1/β-hCG may be developed into a possible approach for the early diagnosis of EP.
      (© 2022. The Author(s).)
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    • الرقم المعرف:
      0 (Chorionic Gonadotropin, beta Subunit, Human)
      0 (Biomarkers)
      EC 2.5.1.18 (GSTO1 protein, human)
      EC 2.5.1.18 (Glutathione Transferase)
    • الموضوع:
      Date Created: 20221121 Date Completed: 20221123 Latest Revision: 20230110
    • الموضوع:
      20230111
    • الرقم المعرف:
      PMC9678856
    • الرقم المعرف:
      10.1038/s41598-022-23374-8
    • الرقم المعرف:
      36411308