Zearalenone Promotes Uterine Development of Weaned Gilts by Interfering with Serum Hormones and Up-Regulating Expression of Estrogen and Progesterone Receptors.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101530765 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6651 (Electronic) Linking ISSN: 20726651 NLM ISO Abbreviation: Toxins (Basel) Subsets: MEDLINE

Original Publication: Basel : MDPI

Zearalenone*/metabolism; Animals ; Female ; Estrogen Receptor alpha/genetics ; Estrogen Receptor alpha/metabolism ; Estrogen Receptor beta/genetics ; Estrogen Receptor beta/metabolism ; Estrogens/metabolism ; Progesterone ; Receptors, Estrogen/genetics ; Receptors, Estrogen/metabolism ; Receptors, Progesterone/genetics ; Receptors, Progesterone/metabolism ; RNA, Messenger/metabolism ; Sus scrofa/metabolism ; Swine ; Uterus

In this study, we aimed to assess the effect of diet ZEA on serum hormones, the location and expression of estrogen receptor ERα/β and progesterone receptor (PR) of the uterus in weaned piglets and to reveal the mechanism underneath. A total of 40 healthy weaned gilts were randomly allocated to basal diet supplemented with 0 (Control), 0.5 (ZEA0.5), 1.0 (ZEA1.0) and 1.5 (ZEA1.5) mg ZEA/kg and fed individually for 35 days. Meanwhile, the porcine endometrial epithelial cells (PECs) were incubated for 24 h with ZEA at 0 (Control), 5 (ZEA5), 20 (ZEA20) and 80 (ZEA80) μmol/L, respectively. The results showed that nutrient apparent digestibility (CP and GE), nutrient apparent availability (ME/GE, BV and NPU), the uterine immunoreactive integrated optic density (IOD), relative mRNA and protein expression of ER-α, ER-β and PR and the relative mRNA and protein expression of ER-α and ER-β in PECs all increased linearly ( p < 0.05) with ZEA. Collectively, ZEA can interfere with the secretion of some reproductive hormones in the serum and promote the expression of estrogen/progesterone receptors in the uterus and PECs. All these indicate that ZEA may promote the development of the uterus in weaned gilts through estrogen receptor pathway.

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Keywords: estrogen receptors; gilts; progesterone receptor; uterus; zearalenone

0 (Estrogen Receptor alpha)
0 (Estrogen Receptor beta)
0 (Estrogens)
4G7DS2Q64Y (Progesterone)
0 (Receptors, Estrogen)
0 (Receptors, Progesterone)
0 (RNA, Messenger)
5W827M159J (Zearalenone)

Date Created: 20221110 Date Completed: 20221116 Latest Revision: 20230125

20230126

PMC9695532

10.3390/toxins14110732

36355982