Formation of Lutein, β-Carotene and Astaxanthin in a Coelastrella sp. Isolate.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, c1995-

Lutein* ; Chlorophyceae*; beta Carotene ; Biofuels ; Nitrates ; Sodium Chloride ; Carotenoids ; Biomass ; Fatty Acids ; Nitrogen

In this study, the effect of media composition, N/P ratio and cultivation strategy on the formation of carotenoids in a Coelastrella sp. isolate was investigated. A two-stage process utilizing different media in the vegetative stage, with subsequent re-suspension in medium without nitrate, was employed to enhance the formation of carotenoids. The optimal growth and carotenoid content (β-carotene and lutein) in the vegetative phase were obtained by cultivation in M-8 and BG11 media. Use of a N/P ratio of 37.5 and low light intensity of 40 μmol m ^-2 s ^-1 (control conditions) led to optimal biomass production of up to 1.31 g L ^-1 . Low concentrations of astaxanthin (maximum of 0.31 wt. %) were accumulated under stress conditions (nitrogen-deficient medium containing 1.5 % of NaCl and light intensity of 500 μmol m ^-2 s ^-1 ), while β-carotene and lutein (combined maximum of 2.12 wt. %) were produced under non-stress conditions. Lipid analysis revealed that palmitic (C16:0) and oleic (C18:1) constituted the main algal fatty acid chains (50.2 ± 2.1% of the total fatty acids), while esterifiable lipids constituted 17.2 ± 0.5% of the biomass by weight. These results suggest that Coelastrella sp. could also be a promising feedstock for biodiesel production.

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DE-PI0000017 U.S.-China Clean Energy Research Center - Advanced Coal Technology Consortium

Keywords: algae; astaxanthin; fatty acids; lutein; pigments; β-carotene

X72A60C9MT (Lutein)
8XPW32PR7I (astaxanthine)
01YAE03M7J (beta Carotene)
0 (Biofuels)
0 (Nitrates)
451W47IQ8X (Sodium Chloride)
36-88-4 (Carotenoids)
0 (Fatty Acids)
N762921K75 (Nitrogen)

Date Created: 20221027 Date Completed: 20221028 Latest Revision: 20221030

20250114

PMC9608941

10.3390/molecules27206950

36296546