Cohesin is required for meiotic spindle assembly independent of its role in cohesion in C. elegans.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101239074 Publication Model: eCollection Cited Medium: Internet ISSN: 1553-7404 (Electronic) Linking ISSN: 15537390 NLM ISO Abbreviation: PLoS Genet Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science, c2005-

Caenorhabditis elegans*/genetics ; Chromosomal Proteins, Non-Histone*/genetics; Animals ; Female ; Cell Cycle Proteins/genetics ; Meiosis/genetics ; Chromatids/genetics ; Chromosome Segregation/genetics ; Spindle Apparatus/genetics ; Cohesins

Accurate chromosome segregation requires a cohesin-mediated physical attachment between chromosomes that are to be segregated apart, and a bipolar spindle with microtubule plus ends emanating from exactly two poles toward the paired chromosomes. We asked whether the striking bipolar structure of C. elegans meiotic chromosomes is required for bipolarity of acentriolar female meiotic spindles by time-lapse imaging of mutants that lack cohesion between chromosomes. Both a spo-11 rec-8 coh-4 coh-3 quadruple mutant and a spo-11 rec-8 double mutant entered M phase with separated sister chromatids lacking any cohesion. However, the quadruple mutant formed an apolar spindle whereas the double mutant formed a bipolar spindle that segregated chromatids into two roughly equal masses. Residual non-cohesive COH-3/4-dependent cohesin on separated sister chromatids of the double mutant was sufficient to recruit haspin-dependent Aurora B kinase, which mediated bipolar spindle assembly in the apparent absence of chromosomal bipolarity. We hypothesized that cohesin-dependent Aurora B might activate or inhibit spindle assembly factors in a manner that would affect their localization on chromosomes and found that the chromosomal localization patterns of KLP-7 and CLS-2 correlated with Aurora B loading on chromosomes. These results demonstrate that cohesin is essential for spindle assembly and chromosome segregation independent of its role in sister chromatid cohesion.
Competing Interests: The authors have declared that no competing interests exist.

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MC_U120097113 United Kingdom MRC_ Medical Research Council; R35 GM136241 United States GM NIGMS NIH HHS

0 (Chromosomal Proteins, Non-Histone)
0 (Cell Cycle Proteins)

Date Created: 20221024 Date Completed: 20221107 Latest Revision: 20231213

20250114

PMC9632809

10.1371/journal.pgen.1010136

36279281