Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

Immunological profiles associated with distinct parasitemic states in volunteers undergoing malaria challenge in Gabon.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: London : Nature Publishing Group, copyright 2011-
    • الموضوع:
      Malaria* ; Malaria Vaccines* ; Malaria, Falciparum*/parasitology; Adult ; Animals ; Gabon ; Humans ; Interferon-gamma ; Parasitemia/parasitology ; Plasmodium falciparum ; Sporozoites ; Volunteers
    • نبذة مختصرة :
      Controlled human malaria infection (CHMI) using cryopreserved non-attenuated Plasmodium falciparum sporozoites (PfSPZ) offers a unique opportunity to investigate naturally acquired immunity (NAI). By analyzing blood samples from 5 malaria-naïve European and 20 African adults with lifelong exposure to malaria, before, 5, and 11 days after direct venous inoculation (DVI) with Sanaria R PfSPZ Challenge, we assessed the immunological patterns associated with control of microscopic and submicroscopic parasitemia. All (5/5) European individuals developed parasitemia as defined by thick blood smear (TBS), but 40% (8/20) of the African individuals controlled their parasitemia, and therefore remained thick blood smear-negative (TBS - Africans). In the TBS - Africans, we observed higher baseline frequencies of CD4 + T cells producing interferon-gamma (IFNγ) that significantly decreased 5 days after PfSPZ DVI. The TBS - Africans, which represent individuals with either very strong and rapid blood-stage immunity or with immunity to liver stages, were stratified into subjects with sub-microscopic parasitemia (TBS - PCR + ) or those with possibly sterilizing immunity (TBS - PCR - ). Higher frequencies of IFNγ + TNF + CD8 + γδ T cells at baseline, which later decreased within five days after PfSPZ DVI, were associated with those who remained TBS - PCR - . These findings suggest that naturally acquired immunity is characterized by different cell types that show varying strengths of malaria parasite control. While the high frequencies of antigen responsive IFNγ + CD4 + T cells in peripheral blood keep the blood-stage parasites to a sub-microscopic level, it is the IFNγ + TNF + CD8 + γδ T cells that are associated with either immunity to the liver-stage, or rapid elimination of blood-stage parasites.
      (© 2022. The Author(s).)
    • References:
      Sci Rep. 2017 Sep 13;7(1):11487. (PMID: 28904345)
      J Immunol. 2016 Dec 15;197(12):4584-4592. (PMID: 27849165)
      PLoS Pathog. 2014 Jan;10(1):e1003864. (PMID: 24415936)
      J Exp Med. 1994 Jul 1;180(1):353-8. (PMID: 7516412)
      Front Immunol. 2017 Aug 23;8:1008. (PMID: 28878775)
      Infect Immun. 2000 Aug;68(8):4399-406. (PMID: 10899836)
      Annu Rev Immunol. 2014;32:157-87. (PMID: 24655294)
      Stem Cells Int. 2019 Dec 6;2019:6348060. (PMID: 31885619)
      Sci Transl Med. 2014 Aug 27;6(251):251ra117. (PMID: 25163477)
      Am J Trop Med Hyg. 2018 Feb;98(2):508-515. (PMID: 29260650)
      J Infect Dis. 2016 Sep 1;214(5):762-71. (PMID: 27296848)
      Nat Commun. 2021 May 4;12(1):2518. (PMID: 33947856)
      BMC Bioinformatics. 2009 Apr 09;10:106. (PMID: 19358741)
      Nat Rev Immunol. 2019 Jul;19(7):457-471. (PMID: 30940932)
      Biostatistics. 2009 Apr;10(2):352-63. (PMID: 19068485)
      Parasitology. 2016 Feb;143(2):208-23. (PMID: 26743398)
      Lancet Infect Dis. 2017 May;17(5):498-509. (PMID: 28216244)
      EBioMedicine. 2019 Jan;39:44-58. (PMID: 30528453)
      J Immunol. 2010 Jun 1;184(11):6043-52. (PMID: 20427769)
      Immunity. 2017 Dec 19;47(6):1197-1209.e10. (PMID: 29195810)
      Infect Immun. 2015 May;83(5):2185-96. (PMID: 25776749)
      Nucleic Acids Res. 2009 Jan;37(Database issue):D539-43. (PMID: 18957442)
      Eur J Immunol. 2011 Jan;41(1):195-201. (PMID: 21182090)
      Nat Immunol. 2021 May;22(5):654-665. (PMID: 33888898)
      Hum Vaccin. 2010 Jan;6(1):97-106. (PMID: 19946222)
      Front Immunol. 2018 Dec 20;9:2973. (PMID: 30619330)
      Clin Microbiol Rev. 2009 Jan;22(1):13-36, Table of Contents. (PMID: 19136431)
      Bioinformatics. 2016 Sep 15;32(18):2847-9. (PMID: 27207943)
      Vaccine. 2017 Dec 4;35(48 Pt B):6720-6726. (PMID: 29042203)
      Parasitol Int. 2019 Apr;69:25-29. (PMID: 30385417)
      J Dermatol Sci. 2011 Aug;63(2):75-82. (PMID: 21708454)
      Nat Immunol. 2008 Jul;9(7):725-32. (PMID: 18563083)
      Scand J Immunol. 1999 Dec;50(6):642-50. (PMID: 10607313)
      Nature. 1961 Nov 25;192:733-7. (PMID: 13880318)
      J Immunol. 2017 Dec 1;199(11):3781-3788. (PMID: 29079696)
      Nat Med. 2016 Jun;22(6):614-23. (PMID: 27158907)
      PLoS Pathog. 2011 Dec;7(12):e1002389. (PMID: 22144890)
      PLoS Comput Biol. 2022 Feb 2;18(2):e1009801. (PMID: 35108259)
    • Grant Information:
      R01GM9987654 United States NH NIH HHS
    • الرقم المعرف:
      0 (Malaria Vaccines)
      82115-62-6 (Interferon-gamma)
    • الموضوع:
      Date Created: 20220803 Date Completed: 20220805 Latest Revision: 20221014
    • الموضوع:
      20231215
    • الرقم المعرف:
      PMC9349185
    • الرقم المعرف:
      10.1038/s41598-022-17725-8
    • الرقم المعرف:
      35922467