In vitro Evaluation of Selective Cytotoxic Activity of Chaerophyllum macropodum Boiss. on Cultured Human SH-SY5Y Neuroblastoma Cells.

Publisher: Springer Country of Publication: United States NLM ID: 100929017 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-3524 (Electronic) Linking ISSN: 10298428 NLM ISO Abbreviation: Neurotox Res Subsets: MEDLINE

Publication: <2009-> : New York : Springer
Original Publication: [Amsterdam?] : Harwood Academic Publishers,

Antineoplastic Agents*/pharmacology ; Antineoplastic Agents*/therapeutic use ; Neuroblastoma*/pathology; Bromides/pharmacology ; Bromides/therapeutic use ; Cell Line, Tumor ; Cell Survival ; Child ; Coumaric Acids/therapeutic use ; Humans ; Lactate Dehydrogenases ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use ; Salicylic Acid/pharmacology ; Salicylic Acid/therapeutic use

Neuroblastoma is the most common solid tumor in children. New treatment approaches are needed because of the harmful side effects and costs of the methods used in the treatment of neuroblastoma. Medicinal and aromatic plants are important for new treatment approaches due to their minimal side effects and economic advantages. Therefore, the present study was carried out to examine the cytotoxic effect of Chaerophyllum macropodum extract on human neuroblastoma (SH-SY5Y) and fibroblast (HDFa) cell lines. 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase release (LDH) assays were used to determine the cytotoxic effect of C. macropodum. The extracts were analyzed for their phenolic content by HPLC-PDA. Major components were determined as 63.600% o-coumaric acid, 15.606% catechine hydrate, 8.713% rosmarinic acid, 4.376% clorogenic acid, and 3.972% salicylic acid. The obtained results from cytotoxicity testing revealed that C. macropodum exerted a significant cytotoxic effect on human neuroblastoma cells at all tested concentrations (p < 0.05). But it did not lead to any cytotoxic potential on human fibroblasts. As a result, the obtained data clearly revealed C. macropodum exerted a selective cytotoxic action on neuroblastoma cells for the first time.
(© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

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Keywords: Anticancer; Apiaceae; Chaerophyllum macropodum; Cytotoxic action; HPLC; Neuroblastoma

0 (Antineoplastic Agents)
0 (Bromides)
0 (Coumaric Acids)
0 (Plant Extracts)
EC 1.1.- (Lactate Dehydrogenases)
O414PZ4LPZ (Salicylic Acid)

Date Created: 20220722 Date Completed: 20220929 Latest Revision: 20220929

20240829

10.1007/s12640-022-00537-z

35867270