Role of an RNA pseudoknot involving the polyA tail in replication of Pepino mosaic potexvirus and related plant viruses.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Solanum lycopersicum*/virology ; Potexvirus*/genetics ; Potexvirus*/physiology ; Virus Replication*; 3' Untranslated Regions/genetics ; Plant Diseases/virology ; RNA, Viral/chemistry

Pepino mosaic virus (PepMV) is a potexvirus of the family Alphaflexiviridae within the order of Tymovirales that threatens tomato production worldwide. PepMV possesses a positive-strand RNA genome with a 5'-methylguanosine cap and a 3'-polyA tail. Previously, using partially-purified viral RNA polymerase important secondary structures within the 3'-untranslated region (UTR) of PepMV RNA were identified. Here we show that an RNA pseudoknot can be formed in the 3'-UTR that includes part of the polyA tail. Using protoplasts, we demonstrate that the pseudoknot is required for replication of PepMV RNA. Mutational analysis and native gel electrophoresis further show that the pseudoknot is stabilized by UAU base triples, as is the human telomerase RNA pseudoknot. The presence of a pseudoknot in several other members of the Alpha- and Betaflexiviridae is supported by covariance analysis and native gel electrophoresis of other potexvirus, capillovirus and trichovirus RNAs. The ubiquitous presence of the pseudoknot in viruses of the Betaflexiviridae, suggests that the pseudoknot is a typical trait of the Betaflexiviridae that may have been adopted by many potexviruses during evolution.
(© 2022. Crown.)

Arch Virol. 2002 Oct;147(10):2009-15. (PMID: 12376761)
Nucleic Acids Res. 1985 Mar 11;13(5):1717-31. (PMID: 4000943)
Nucleic Acids Res. 2022 Jan 25;50(2):1052-1068. (PMID: 34928389)
Virus Res. 2014 Sep 22;190:110-7. (PMID: 25051146)
Mol Cell. 2005 Mar 4;17(5):671-82. (PMID: 15749017)
Wiley Interdiscip Rev RNA. 2020 Nov;11(6):e1598. (PMID: 32441456)
J Virol. 1996 Aug;70(8):5266-71. (PMID: 8764036)
Virus Res. 2012 Aug;167(2):267-72. (PMID: 22617023)
Plant Methods. 2011 Mar 11;7:6. (PMID: 21396092)
Mol Plant Pathol. 2010 Mar;11(2):179-89. (PMID: 20447268)
Infect Genet Evol. 2011 Mar;11(2):489-95. (PMID: 21232632)
PLoS Pathog. 2012;8(5):e1002726. (PMID: 22654666)
Wiley Interdiscip Rev RNA. 2015 Jan-Feb;6(1):111-28. (PMID: 25146348)
J Mol Biol. 2003 Feb 21;326(3):701-20. (PMID: 12581634)
New Phytol. 2014 Apr;202(1):233-246. (PMID: 24372390)
Virol J. 2013 Jun 24;10:208. (PMID: 23800142)
RNA. 2002 Jan;8(1):110-22. (PMID: 11873757)
J Virol. 1999 Apr;73(4):2703-9. (PMID: 10074116)
J Virol. 2002 Jun;76(12):6114-20. (PMID: 12021344)
Nucleic Acids Res. 2000 Jan 1;28(1):201-4. (PMID: 10592225)
Nucleic Acids Res. 2003 Jul 1;31(13):3406-15. (PMID: 12824337)
Virology. 1994 Aug 1;202(2):707-14. (PMID: 7518162)
J Gen Virol. 2005 Jun;86(Pt 6):1827-1833. (PMID: 15914862)
Science. 2010 Nov 26;330(6008):1244-7. (PMID: 21109672)
Viruses. 2021 Apr 09;13(4):. (PMID: 33918611)
Virology. 1992 Aug;189(2):609-17. (PMID: 1641981)

0 (3' Untranslated Regions)
0 (RNA, Viral)

Pepino mosaic virus

Date Created: 20220707 Date Completed: 20220711 Latest Revision: 20221207

20221213

PMC9262919

10.1038/s41598-022-15598-5

35798958