Modulation of GmFAD3 expression alters abiotic stress responses in soybean.

Publisher: Kluwer Academic Country of Publication: Netherlands NLM ID: 9106343 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-5028 (Electronic) Linking ISSN: 01674412 NLM ISO Abbreviation: Plant Mol Biol Subsets: MEDLINE

Publication: Dordrecht : Kluwer Academic
Original Publication: The Hague ; Boston : Martinus Nijhoff/Dr. W. Junk, 1981-

Gene Expression Regulation, Plant* ; Glycine max*/physiology; Comovirus ; Droughts ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Plants, Genetically Modified/genetics ; Stress, Physiological/genetics

Key Message: This study focused on enhancing resilience of soybean crops to drought and salinity stresses by overexpression of GmFAD3A gene, which plays an important role in modulating membrane fluidity and ultimately influence plants response to various abiotic stresses. Fatty acid desaturases (FADs) are a class of enzymes that mediate desaturation of fatty acids by introducing double bonds. They play an important role in modulating membrane fluidity in response to various abiotic stresses. However, a comprehensive analysis of GmFAD3 in drought and salinity stress tolerance in soybean is lacking. We used bean pod mottle virus (BPMV)-based vector for achieving rapid and efficient overexpression as well as silencing of Omega-3 Fatty Acid Desaturase gene from Glycine max (GmFAD3) to assess the functional role of GmFAD3 in abiotic stress responses in soybean. Higher levels of recombinant BPMV-GmFAD3A transcripts were detected in overexpressing soybean plants. Overexpression of GmFAD3A in soybean resulted in increased levels of jasmonic acid and higher expression of GmWRKY54 as compared to mock-inoculated, vector-infected and FAD3-silenced soybean plants under drought and salinity stress conditions. The GmFAD3A-overexpressing plants showed higher levels of chlorophyll content, efficient photosystem-II, relative water content, transpiration rate, stomatal conductance, proline content and also cooler canopy under drought and salinity stress conditions as compared to mock-inoculated, vector-infected and FAD3-silenced soybean plants. Results from the current study revealed that GmFAD3A-overexpressing soybean plants exhibited tolerance to drought and salinity stresses. However, soybean plants silenced for GmFAD3 were vulnerable to drought and salinity stresses.
(© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)

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NASF/GTR-6035/2018-19 Indian Council of Agricultural Research-National Agriculture Science Fund

Keywords: Bean pod mottle virus; Drought stress; Fatty acid desaturase; Gene silencing; Jasmonic acid; Overexpression; Salinity stress; WRKY transcription factor

0 (Plant Proteins)

Bean pod mottle virus

Date Created: 20220702 Date Completed: 20220914 Latest Revision: 20231213

20231215

10.1007/s11103-022-01295-4

35779188