The transcription factor OsMYBc and an E3 ligase regulate expression of a K+ transporter during salt stress.

Publisher: American Society of Plant Biologists Country of Publication: United States NLM ID: 0401224 Publication Model: Print Cited Medium: Internet ISSN: 1532-2548 (Electronic) Linking ISSN: 00320889 NLM ISO Abbreviation: Plant Physiol Subsets: MEDLINE

Publication: : [Rockville, MD] : American Society of Plant Biologists
Original Publication: Lancaster, Pa., American Society of Plant Physiologists.

Cation Transport Proteins*/genetics ; Cation Transport Proteins*/metabolism ; Oryza*/genetics ; Oryza*/metabolism ; Plant Proteins*/genetics ; Plant Proteins*/metabolism ; Plants, Genetically Modified*/genetics ; Plants, Genetically Modified*/metabolism ; Salt Stress*/genetics ; Symporters*/genetics ; Symporters*/metabolism ; Transcription Factors*/genetics ; Transcription Factors*/metabolism ; Ubiquitin-Protein Ligases*/metabolism; Gene Expression Regulation, Plant ; Potassium/metabolism ; Sodium/metabolism

Sodium (Na+) and potassium (K+) homeostasis is essential for plant survival in saline soils. A member of the High-Affinity K+ Transporter (HKT) family in rice (Oryza sativa), OsHKT1;1, is a vital regulator of Na+ exclusion from shoots and is bound by a MYB transcription factor (OsMYBc). Here, we generated transgenic rice lines in the oshkt1;1 mutant background for genetic complementation using genomic OsHKT1;1 containing a native (Com) or mutated (mCom) promoter that cannot be bound by OsMYBc. In contrast to wild-type (WT) or Com lines, the mCom lines were not able to recover the salt-sensitive phenotype of oshkt1;1. The OsMYBc-overexpressing plants were more tolerant to salt stress than WT plants. A yeast two-hybrid screen using the OsMYBc N-terminus as bait identified a rice MYBc stress-related RING finger protein (OsMSRFP). OsMSRFP is an active E3 ligase that ubiquitinated OsMYBc in vitro and mediated 26S proteasome-mediated degradation of OsMYBc under semi-in vitro and in vivo conditions. OsMSRFP attenuated OsMYBc-mediated OsHKT1;1 expression, and knockout of OsMSRFP led to rice salt tolerance. These findings uncover a regulatory mechanism of salt response that fine-tunes OsHKT1;1 transcription by ubiquitination of OsMYBc.
(© American Society of Plant Biologists 2022. All rights reserved. For permissions, please email: journals.permissions@oup.com.)

Trends Plant Sci. 2014 Jun;19(6):371-9. (PMID: 24630845)
Plant Physiol. 2017 Aug;174(4):2515-2531. (PMID: 28626006)
Nat Commun. 2016 Sep 12;7:12525. (PMID: 27615387)
Plant Cell. 2007 Jun;19(6):1912-29. (PMID: 17573536)
Nature. 2002 Sep 12;419(6903):167-70. (PMID: 12226665)
Plant Cell Environ. 2021 Sep;44(9):2951-2965. (PMID: 34008219)
Plant Cell. 2014 Sep;26(9):3630-45. (PMID: 25248553)
Nat Biotechnol. 2012 Mar 11;30(4):360-4. (PMID: 22407351)
Plant J. 2000 Jun;22(6):543-51. (PMID: 10886774)
Nat Rev Mol Cell Biol. 2009 Jun;10(6):385-97. (PMID: 19424292)
Plant Physiol. 2020 Jan;182(1):584-596. (PMID: 31690708)
Plant Cell. 2018 Jan;30(1):228-244. (PMID: 29237723)
Plant Physiol. 2009 Feb;149(2):916-28. (PMID: 19036832)
Plant Cell. 2003 Aug;15(8):1771-80. (PMID: 12897251)
Plant Physiol. 2011 Jun;156(2):550-63. (PMID: 21478367)
Plant Physiol. 2019 Jun;180(2):1101-1118. (PMID: 30894419)
Plants (Basel). 2021 Sep 24;10(10):. (PMID: 34685816)
BMC Plant Biol. 2013 Feb 27;13:32. (PMID: 23445750)
Plant Physiol. 2017 Jul;174(3):1837-1849. (PMID: 28487477)
Planta. 2018 Apr;247(4):925-939. (PMID: 29285618)
PLoS Genet. 2017 Jun 5;13(6):e1006823. (PMID: 28582424)
Plant J. 2017 Aug;91(4):657-670. (PMID: 28488420)
Annu Rev Plant Biol. 2017 Apr 28;68:405-434. (PMID: 28226230)
Plant Physiol. 2016 Jul;171(3):2112-26. (PMID: 27208305)
PLoS One. 2012;7(7):e39865. (PMID: 22808069)
Plant Cell. 2013 Jun;25(6):2187-201. (PMID: 23735294)
J Integr Plant Biol. 2013 Jun;55(6):504-13. (PMID: 23480486)
Plant J. 2013 Mar;73(6):993-1005. (PMID: 23240817)
Annu Rev Genet. 2009;43:265-85. (PMID: 19686081)
Plant Mol Biol. 2015 Mar;87(4-5):441-58. (PMID: 25667045)
Proc Natl Acad Sci U S A. 2020 Nov 3;117(44):27694-27702. (PMID: 33077597)
Annu Rev Plant Biol. 2020 Apr 29;71:403-433. (PMID: 32167791)
Plant Cell. 2015 Jan;27(1):214-27. (PMID: 25616872)
Trends Plant Sci. 2010 Oct;15(10):573-81. (PMID: 20674465)
Plant Physiol. 2009 May;150(1):463-81. (PMID: 19286935)
EMBO J. 2007 Jun 20;26(12):3003-14. (PMID: 17541409)
Plant Physiol. 2015 Jul;168(3):1076-90. (PMID: 25991736)
New Phytol. 2012 Oct;196(1):13-28. (PMID: 22897362)
New Phytol. 2017 May;214(3):1172-1187. (PMID: 28157263)
Plant Physiol. 2013 Apr;161(4):1615-33. (PMID: 23391577)
BMC Plant Biol. 2016 Jan 19;16:22. (PMID: 26786707)
Nat Commun. 2013;4:1476. (PMID: 23403577)
Plant Physiol. 2019 Feb;179(2):616-629. (PMID: 30498025)
Plant J. 2008 Nov;56(3):505-16. (PMID: 18643980)
FEBS Lett. 2013 Aug 19;587(16):2584-90. (PMID: 23831064)
Plant J. 2009 Mar;57(5):798-809. (PMID: 18980647)
Plant Physiol. 2009 Aug;150(4):1955-71. (PMID: 19482918)
Mol Plant. 2016 Jan 4;9(1):126-135. (PMID: 26410794)
Nat Biotechnol. 2015 May;33(5):445-9. (PMID: 25798936)
Annu Rev Plant Biol. 2008;59:651-81. (PMID: 18444910)
New Phytol. 2018 Jan;217(2):523-539. (PMID: 29205383)
Plant Physiol. 2018 Jul;177(3):875-895. (PMID: 29691301)
Nat Protoc. 2006;1(6):2796-802. (PMID: 17406537)
Annu Rev Plant Biol. 2002;53:247-73. (PMID: 12221975)
Nat Rev Genet. 2015 Apr;16(4):237-51. (PMID: 25752530)
Nat Genet. 2005 Oct;37(10):1141-6. (PMID: 16155566)
Curr Opin Plant Biol. 2016 Oct;33:126-132. (PMID: 27450430)

0 (Cation Transport Proteins)
0 (HKT1 protein, plant)
0 (Plant Proteins)
0 (Symporters)
0 (Transcription Factors)
9NEZ333N27 (Sodium)
EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RWP5GA015D (Potassium)

Date Created: 20220613 Date Completed: 20220908 Latest Revision: 20230614

20230614

PMC9434319

10.1093/plphys/kiac283

35695778