Quantitative glycoproteomics of human milk and association with atopic disease.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science

Breast Feeding* ; Hypersensitivity, Immediate*/epidemiology ; Milk, Human*/chemistry; Child ; Ethnicity ; Female ; Glycopeptides ; Glycoproteins ; Humans ; Life Style ; Milk Proteins ; New York ; Proteomics

The prevalence of allergic diseases and asthma is increasing rapidly worldwide, with environmental and lifestyle behaviors implicated as a reason. Epidemiological studies have shown that children who grow up on farms are at lower risk of developing childhood atopic disease, indicating the presence of a protective "farm effect". The Old Order Mennonite (OOM) community in Upstate New York have traditional, agrarian lifestyles, a low rate of atopic disease, and long periods of exclusive breastfeeding. Human milk proteins are heavily glycosylated, although there is a paucity of studies investigating the milk glycoproteome. In this study, we have used quantitative glycoproteomics to compare the N-glycoprotein profiles of 54 milk samples from Rochester urban/suburban and OOM mothers, two populations with different lifestyles, exposures, and risk of atopic disease. We also compared N-glycoprotein profiles according to the presence or absence of atopic disease in the mothers and, separately, the children. We identified 79 N-glycopeptides from 15 different proteins and found that proteins including immunoglobulin A1, polymeric immunoglobulin receptor, and lactotransferrin displayed significant glycan heterogeneity. We found that the abundances of 38 glycopeptides differed significantly between Rochester and OOM mothers and also identified four glycopeptides with significantly different abundances between all comparisons. These four glycopeptides may be associated with the development of atopic disease. The findings of this study suggest that the differential glycosylation of milk proteins could be linked to atopic disease.
Competing Interests: The authors have declared that no competing interests exist.

Mucosal Immunol. 2018 May;11(3):643-653. (PMID: 29346344)
J Proteome Res. 2011 Apr 1;10(4):1746-54. (PMID: 21361340)
Allergy. 2021 Nov;76(11):3489-3503. (PMID: 33905556)
Allergy. 2017 Dec;72(12):1936-1943. (PMID: 28449362)
J Agric Food Chem. 2017 Nov 29;65(47):10360-10367. (PMID: 29110469)
Glycobiology. 2008 Apr;18(4):339-49. (PMID: 18272656)
Glycobiology. 2015 Dec;25(12):1323-4. (PMID: 26543186)
Nat Rev Immunol. 2010 Dec;10(12):861-8. (PMID: 21060319)
Anal Bioanal Chem. 2017 Jan;409(2):589-606. (PMID: 27796459)
Nat Commun. 2020 Jan 8;11(1):120. (PMID: 31913287)
Lancet. 2001 Oct 6;358(9288):1129-33. (PMID: 11597666)
Nutrients. 2019 Aug 08;11(8):. (PMID: 31398857)
Ann Allergy Asthma Immunol. 2016 Nov;117(5):562-563.e1. (PMID: 27612936)
J Allergy Clin Immunol. 2005 Jun;115(6):1238-48. (PMID: 15940141)
Annu Rev Immunol. 1994;12:63-84. (PMID: 8011293)
PLoS One. 2018 Mar 29;13(3):e0195006. (PMID: 29596458)
World Allergy Organ J. 2014 May 19;7(1):12. (PMID: 24940476)
Ann Allergy Asthma Immunol. 2020 Mar;124(3):292-294.e2. (PMID: 31923545)
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Dec 15;876(2):252-6. (PMID: 19019742)
Food Funct. 2018 Feb 21;9(2):1163-1172. (PMID: 29363704)
PLoS One. 2015 Mar 23;10(3):e0122234. (PMID: 25798592)
Proteomics. 2008 Sep;8(18):3833-47. (PMID: 18780401)

R21 TR002516 United States TR NCATS NIH HHS

0 (Glycopeptides)
0 (Glycoproteins)
0 (Milk Proteins)

Date Created: 20220513 Date Completed: 20220517 Latest Revision: 20220629

20221213

PMC9106177

10.1371/journal.pone.0267967

35559953