Genetic engineering of the branched-chain fatty acid biosynthesis pathway to enhance surfactin production from Bacillus subtilis.

Publisher: Wiley-Blackwell Country of Publication: United States NLM ID: 8609465 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1470-8744 (Electronic) Linking ISSN: 08854513 NLM ISO Abbreviation: Biotechnol Appl Biochem Subsets: MEDLINE

Publication: Jan. 2011- : Malden : Wiley-Blackwell
Original Publication: San Diego : Academic Press, [cl986]-

Bacillus subtilis*/genetics ; Bacillus subtilis*/metabolism ; Fatty Acids*/metabolism; Genetic Engineering ; Protein Isoforms/genetics ; Protein Isoforms/metabolism ; Lipopeptides/genetics ; Lipopeptides/metabolism ; Peptides, Cyclic/genetics ; Peptides, Cyclic/metabolism

Surfactin, which is composed of a β-hydroxy fatty acid chain and a peptide ring, has drawn considerable attention due to its potential applications in the biomedicine, bioremediation, and petroleum industries. However, the low yield of surfactin from wild strains still restricts its industrial applications. In this study, eight genes relevant to the fatty acid biosynthesis pathway were targeted to enhance surfactin production, and high surfactin-yielding strains with potential industrial applications were obtained. When ldeHA and acc were co-overexpressed, the surfactin yield of recombinant strains TDS8 and TPS8 increased to 1.55- and 1.19-fold of their parental strains, respectively, again proving that the conversion of acetyl-coenzyme A (CoA) to malonyl-CoA is the rate-limiting step in fatty acid biosynthesis. Furthermore, changes in surfactin isoforms of recombinant strain TPS8 suggest that the fatty acid precursor synthesis pathway can be modified to improve the proportion of different isoforms. In addition, the deletion of lpdV, which is responsible for the conversion of α-ketoacyl-CoA precursors, resulted in a sharp decrease in surfactin production, further demonstrating the importance of branched-chain fatty acid biosynthesis in surfactin production. This work will facilitate the design and construction of more efficiently engineered strains for surfactin production and further extend industrial applications.
(© 2022 International Union of Biochemistry and Molecular Biology, Inc.)

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Keywords: Bacillus subtilis; fatty acid; gene expression; metabolic engineering; precursor biosynthesis; surfactin

0 (Fatty Acids)
0 (Protein Isoforms)
0 (Lipopeptides)
0 (Peptides, Cyclic)

Date Created: 20220414 Date Completed: 20230223 Latest Revision: 20230223

20250114

10.1002/bab.2346

35419893