Transcriptomic Analysis of the Host Response to Mild and Severe CTV Strains in Naturally Infected Citrus sinensis Orchards.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Citrus sinensis/*virology ; Closterovirus/*pathogenicity ; Gene Expression Profiling/*methods ; Plant Proteins/*genetics ; Plant Viruses/*pathogenicity ; Viral Proteins/*genetics; Citrus sinensis/genetics ; Closterovirus/genetics ; Disease Resistance ; Gene Expression Regulation, Plant ; Gene Expression Regulation, Viral ; Mexico ; Plant Diseases/genetics ; Plant Diseases/virology ; Plant Viruses/genetics ; RNA-Seq ; Virulence

Citrus tristeza virus (CTV) is an important threat to the global citrus industry, causing severe economic losses worldwide. The disease management strategies are focused on vector control, tree culling, and the use of resistant varieties and rootstocks. Sweet orange ( Citrus sinensis ) trees showing either severe or mild CTV symptoms have been observed in orchards in Veracruz, Mexico, and were probably caused by different virus strains. To understand these symptomatic differences, transcriptomic analyses were conducted using asymptomatic trees. CTV was confirmed to be associated with infected plants, and mild and severe strains were successfully identified by a polymorphism in the coat protein (CP) encoding gene. RNA-Seq analysis revealed more than 900 significantly differentially expressed genes in response to mild and severe strains, with some overlapping genes. Importantly, multiple sequence reads corresponding to Citrus exocortis viroid and Hop stunt viroid were found in severe symptomatic and asymptomatic trees, but not in plants with mild symptoms. The differential gene expression profiling obtained in this work provides an overview of molecular behavior in naturally CTV-infected trees. This work may contribute to our understanding of citrus-virus interaction in more natural settings, which can help develop strategies for integrated crop management.

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2017, 2018 Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria (SENASICA) Mexico

Keywords: Citrus sinensis; Citrus tristeza virus; RNA-Seq; differential gene expression; mild/severe strain

0 (Plant Proteins)
0 (Viral Proteins)

Citrus tristeza virus; Hop stunt viroid

Date Created: 20220310 Date Completed: 20220329 Latest Revision: 20220329

20221213

PMC8910659

10.3390/ijms23052435

35269578