Cyanidin attenuates the apoptosis of rat nucleus pulposus cells and the degeneration of intervertebral disc via the JAK2/STAT3 signal pathway in vitro and in vivo .

Publisher: Taylor & Francis Country of Publication: England NLM ID: 9812552 Publication Model: Print Cited Medium: Internet ISSN: 1744-5116 (Electronic) Linking ISSN: 13880209 NLM ISO Abbreviation: Pharm Biol Subsets: MEDLINE

Publication: [London] : Taylor & Francis
Original Publication: Lisse, the Netherlands : Swets & Zeitlinger, c1998-

Anthocyanins/*pharmacology ; Apoptosis/*drug effects ; Intervertebral Disc Degeneration/*prevention & control ; Nucleus Pulposus/*drug effects; Animals ; Anthocyanins/administration & dosage ; Dose-Response Relationship, Drug ; Inhibitory Concentration 50 ; Interleukin-1beta/administration & dosage ; Janus Kinase 2/metabolism ; Male ; Rats ; Rats, Sprague-Dawley ; STAT3 Transcription Factor/metabolism ; Signal Transduction/drug effects

Context: Cyanidin has been shown to have therapeutic potential in osteoarthritis. However, it is unclear whether cyanidin prevents the progression of intervertebral disc degeneration (IVDD).
Objective: This study evaluates the effects of cyanidin on IVDD in vitro and in vivo .
Materials and Methods: Nucleus pulposus cells (NPCs) isolated from lumbar IVD of 4-week-old male Sprague-Dawley (SD) rats were exposed to 20 ng/mL IL-1β, and then treated with different doses (0-120 µM) of cyanidin for 24 h. SD rats were classified into three groups ( n  = 8) and treated as follows: control (normal saline), IVDD (vehicle), IVDD + cyanidin (50 mg/kg). Cyanidin was administered intraperitoneally for 8 weeks.
Results: The IC 50 of cyanidin for NPCs was 94.78 µM, and cyanidin had no toxicity at concentrations up to 500 mg/kg in SD rats. Cyanidin inhibited the apoptosis of NPCs induced by IL-1β (12.73 ± 0.61% vs. 18.54 ± 0.60%), promoted collagen II (0.82-fold) and aggrecan (0.81-fold) expression, while reducing MMP-13 (1.02-fold) and ADAMTS-5 (1.40-fold) expression. Cyanidin increased the formation of autophagosomes in IL-1β-induced NPCs, and promoted LC3II/LC3I (0.83-fold) and beclin-1 (0.85-fold) expression, which could be reversed by chloroquine. Cyanidin inhibited the phosphorylation of JAK2 (0.47-fold) and STAT3 (0.53-fold) in IL-1β-induced NPCs. The effects of cyanidin could be enhanced by AG490. Furthermore, cyanidin mitigated disc degeneration in IVDD rats in vivo .
Discussion and Conclusions: Cyanidin improved the function of NPCs in IVDD by regulating the JAK2/STAT3 pathway, which may provide a novel alternative strategy for IVDD. The mechanism of cyanidin improving IVDD still needs further work for in-depth investigation.

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Keywords: Extracellular matrix degeneration; autophagy; chloroquine; natural polyphenolic compounds

0 (Anthocyanins)
0 (Interleukin-1beta)
0 (STAT3 Transcription Factor)
0 (Stat3 protein, rat)
7732ZHU564 (cyanidin)
EC 2.7.10.2 (Jak2 protein, rat)
EC 2.7.10.2 (Janus Kinase 2)

Date Created: 20220217 Date Completed: 20220310 Latest Revision: 20220501

20250114

PMC8856032

10.1080/13880209.2022.2035773

35175176