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Immunoexpression of PTEN, HER2/neu, and Ki-67 in endoscopic gastric carcinoma biopsies; their correlation with histological subtypes and one-year survival.

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  • المؤلفون: Acharya S;Acharya S; Thakur B; Thakur B; Mittal R; Mittal R
  • المصدر:
    Indian journal of pathology & microbiology [Indian J Pathol Microbiol] 2022 Jan-Mar; Vol. 65 (1), pp. 29-34.
  • نوع النشر :
    Journal Article
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Medknow Country of Publication: India NLM ID: 7605904 Publication Model: Print Cited Medium: Internet ISSN: 0974-5130 (Electronic) Linking ISSN: 03774929 NLM ISO Abbreviation: Indian J Pathol Microbiol Subsets: MEDLINE
    • بيانات النشر:
      Publication: Mumbai : Medknow
      Original Publication: Chandigarh : Indian Association of Pathologists & Microbiologists.
    • الموضوع:
      Gene Expression*/immunology; Ki-67 Antigen/*genetics ; PTEN Phosphohydrolase/*genetics ; Receptor, ErbB-2/*genetics ; Stomach Neoplasms/*classification ; Stomach Neoplasms/*genetics; Adenocarcinoma/genetics ; Adenocarcinoma/immunology ; Aged ; Aged, 80 and over ; Biomarkers, Tumor/immunology ; Biopsy ; Endoscopy, Gastrointestinal/methods ; Female ; Humans ; Immunochemistry/methods ; Ki-67 Antigen/immunology ; Male ; Middle Aged ; PTEN Phosphohydrolase/immunology ; Receptor, ErbB-2/immunology ; Stomach/pathology ; Stomach Neoplasms/diagnosis ; Stomach Neoplasms/mortality ; Survival Analysis
    • نبذة مختصرة :
      Background: Gastric carcinoma is a major cause of cancer-related morbidity and mortality worldwide. Gastric neoplasms arise from genetic and epigenetic changes in various genes. Present study evaluates the immunoexpression of PTEN, HER2/neu, and Ki-67 in endoscopic gastric carcinoma biopsies and correlates the expression of these proteins with clinicopathological features.
      Material and Methods: Adequate endoscopic biopsies of 27 cases of gastric carcinoma were evaluated for World Health Organization (WHO) and Lauren's classification subtypes along with HER2/neu, PTEN, and Ki-67 immunoexpression. HER2/neu immunostaining was scored as proposed in the Trastuzumab for gastric cancer (ToGA) trial while PTEN staining and downregulation were assessed using an immunoreactive score. The cut-off for Ki-67 expression was taken as 90 th percentile of the values in adjacent non-neoplastic tissue. All statistical analysis was done at 5% level of significance with SPSS v22 statistical software.
      Results: Tubular adenocarcinoma was the commonest WHO histological subtype and 56% of cases were of intestinal type as per Lauren's classification. 55.6% of cases showed a complete loss of PTEN expression in neoplastic tissue. 17 of the 19 cases with adjacent non-neoplastic tissue showed PTEN downregulation in neoplastic tissue. 81.5% of cases had a high Ki-67 index and HER2/neu overexpression was noted in 36% of cases. All the four cases who died had high Ki-67 proliferation indices; 3 patients had loss of PTEN expression and HER2/neu overexpression.
      Conclusion: We conclude that these immunomarkers can play important role in the behavior of gastric carcinomas and can be targeted for new therapies.
      Competing Interests: None
    • Contributed Indexing:
      Keywords: Carcinomas; HER2/neu; IRS; Ki-67; PTEN; gastric
    • الرقم المعرف:
      0 (Biomarkers, Tumor)
      0 (Ki-67 Antigen)
      EC 2.7.10.1 (ERBB2 protein, human)
      EC 2.7.10.1 (Receptor, ErbB-2)
      EC 3.1.3.67 (PTEN Phosphohydrolase)
      EC 3.1.3.67 (PTEN protein, human)
    • الموضوع:
      Date Created: 20220125 Date Completed: 20220223 Latest Revision: 20220430
    • الموضوع:
      20221213
    • الرقم المعرف:
      10.4103/IJPM.IJPM_299_20
    • الرقم المعرف:
      35074962