The life cycle-dependent transcriptional profile of the obligate intracellular amoeba symbiont Amoebophilus asiaticus.

Publisher: Oxford University Press Country of Publication: England NLM ID: 8901229 Publication Model: Print Cited Medium: Internet ISSN: 1574-6941 (Electronic) Linking ISSN: 01686496 NLM ISO Abbreviation: FEMS Microbiol Ecol Subsets: MEDLINE

Publication: 2015- : Oxford Oxford University Press
Original Publication: [Amsterdam] : Elsevier Science Publishers on behalf of the Federation of European Microbiological Societies, [1985-

Amoeba*/genetics; Animals ; Bacteria/genetics ; Bacteroidetes/genetics ; Life Cycle Stages ; Symbiosis

Free-living amoebae often harbor obligate intracellular bacterial symbionts. Amoebophilus (A.) asiaticus is a representative of a lineage of amoeba symbionts in the phylum Bacteroidota. Here, we analyse the transcriptome of A. asiaticus strain 5a2 at four time points during its infection cycle and replication within the Acanthamoeba host using RNA sequencing. Our results reveal a dynamic transcriptional landscape throughout different A. asiaticus life cycle stages. Many intracellular bacteria and pathogens utilize eukaryotic-like proteins (ELPs) for host cell interaction and the A. asiaticus 5a2 genome shows a particularly high abundance of ELPs. We show the expression of all genes encoding ELPs and found many ELPs to be differentially expressed. At the replicative stage of A. asiaticus, ankyrin repeat proteins and tetratricopeptide/Sel1-like repeat proteins were upregulated. At the later time points, high expression levels of a type 6 secretion system that likely prepares for a new infection cycle after lysing its host, were found. This study reveals comprehensive insights into the intracellular lifestyle of A. asiaticus and highlights candidate genes for host cell interaction. The results from this study have implications for other intracellular bacteria such as other amoeba-associated bacteria and the arthropod symbionts Cardinium forming the sister lineage of A. asiaticus.
(© The Author(s) 2022. Published by Oxford University Press on behalf of FEMS.)

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Keywords: Amoebophilus asiaticus; amoeba; eukaryotic-like proteins; symbiont; transcriptome; type 6 secretion system

Date Created: 20220109 Date Completed: 20220407 Latest Revision: 20220407

20240829

PMC8831229

10.1093/femsec/fiac001

34999767