Padlock Probe-Based Generation of DNAzymes for the Colorimetric Detection of Antibiotic Resistance Genes.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Drug Resistance, Bacterial*; Bacteria/*genetics ; Bacterial Proteins/*analysis ; DNA, Catalytic/*metabolism ; Multiplex Polymerase Chain Reaction/*methods ; Sepsis/*microbiology; Acinetobacter baumannii/genetics ; Bacterial Proteins/metabolism ; Biosensing Techniques ; Colorimetry ; DNA Probes/metabolism ; DNA, Catalytic/chemistry ; Enterobacter cloacae/genetics ; Enterococcus faecium/genetics ; G-Quadruplexes ; Humans ; Klebsiella pneumoniae/genetics ; Nucleic Acid Amplification Techniques ; Proof of Concept Study ; Sepsis/genetics

The increasing emergence of multidrug- and pan-resistant pathogens requires rapid and cost-efficient diagnostic tools to contain their further spread in healthcare facilities and the environment. The currently established diagnostic technologies are of limited utility for efficient infection control measures because they are either cultivation-based and time-consuming or require sophisticated assays that are expensive. Furthermore, infectious diseases are unfortunately most problematic in countries with low-resource settings in their healthcare systems. In this study, we developed a cost-efficient detection technology that uses G-quadruplex DNAzymes to convert a chromogenic substrate resulting in a color change in the presence of antibiotic resistance genes. The assay is based on padlock probes capable of high-multiplex reactions and targets 27 clinically relevant antibiotic resistance genes associated with sepsis. In addition to an experimental proof-of-principle using synthetic target DNA, the assay was evaluated with multidrug-resistant clinical isolates.

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686647 European Commission

Keywords: DNAzyme; G-quadruplex; antibiotic resistance; colorimetric detection; padlock probe

0 (Bacterial Proteins)
0 (DNA Probes)
0 (DNA, Catalytic)

Date Created: 20211224 Date Completed: 20220128 Latest Revision: 20220128

20221213

PMC8715668

10.3390/ijms222413654

34948456