Mouse Acidic Chitinase Effectively Degrades Random-Type Chitosan to Chitooligosaccharides of Variable Lengths under Stomach and Lung Tissue pH Conditions.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, c1995-

Hydrogen-Ion Concentration*; Chitinases/*metabolism ; Chitosan/*metabolism ; Lung/*metabolism ; Oligosaccharides/*metabolism ; Stomach/*metabolism; Animals ; Chitinases/chemistry ; Chitosan/chemistry ; Hydrolysis ; Mice ; Oligosaccharides/chemistry ; Organ Specificity ; Substrate Specificity ; X-Ray Diffraction

Chitooligosaccharides exhibit several biomedical activities, such as inflammation and tumorigenesis reduction in mammals. The mechanism of the chitooligosaccharides' formation in vivo has been, however, poorly understood. Here we report that mouse acidic chitinase (Chia), which is widely expressed in mouse tissues, can produce chitooligosaccharides from deacetylated chitin (chitosan) at pH levels corresponding to stomach and lung tissues. Chia degraded chitin to produce N -acetyl-d-glucosamine (GlcNAc) dimers. The block-type chitosan (heterogenous deacetylation) is soluble at pH 2.0 (optimal condition for mouse Chia) and was degraded into chitooligosaccharides with various sizes ranging from di- to nonamers. The random-type chitosan (homogenous deacetylation) is soluble in water that enables us to examine its degradation at pH 2.0, 5.0, and 7.0. Incubation of these substrates with Chia resulted in the more efficient production of chitooligosaccharides with more variable sizes was from random-type chitosan than from the block-type form of the molecule. The data presented here indicate that Chia digests chitosan acquired by homogenous deacetylation of chitin in vitro and in vivo. The degradation products may then influence different physiological or pathological processes. Our results also suggest that bioactive chitooligosaccharides can be obtained conveniently using homogenously deacetylated chitosan and Chia for various biomedical applications.

Sci Rep. 2016 Nov 24;6:37756. (PMID: 27883045)
Biomacromolecules. 2009 May 11;10(5):1100-5. (PMID: 19334783)
Carbohydr Polym. 2016 Mar 15;138:259-64. (PMID: 26794761)
Food Chem Toxicol. 2009 Aug;47(8):1864-71. (PMID: 19427889)
J Allergy Clin Immunol. 2018 Aug;142(2):364-369. (PMID: 29959948)
Genetics. 2007 Oct;177(2):959-70. (PMID: 17720922)
Int J Biol Macromol. 2019 Aug 1;134:882-890. (PMID: 31108147)
Poult Sci. 2017 Jan 1;96(1):27-34. (PMID: 27520069)
PLoS One. 2016 Oct 7;11(10):e0164367. (PMID: 27716783)
J Funct Biomater. 2015 Jan 14;6(1):33-49. (PMID: 25594943)
Pharmacol Res. 2008 Mar;57(3):247-52. (PMID: 18353673)
Mol Biol Evol. 2016 Dec;33(12):3183-3193. (PMID: 27702777)
Mar Drugs. 2010 Jun 28;8(7):1988-2012. (PMID: 20714419)
Carbohydr Polym. 2017 Nov 15;176:177-186. (PMID: 28927596)
Int Immunopharmacol. 2011 Jan;11(1):121-7. (PMID: 21059391)
Biochim Biophys Acta. 2007 Apr;1770(4):495-505. (PMID: 17240531)
Biomacromolecules. 2011 Sep 12;12(9):3285-90. (PMID: 21790136)
Carbohydr Polym. 2015 Nov 5;132:304-10. (PMID: 26256353)
PLoS One. 2013 Nov 11;8(11):e78669. (PMID: 24244337)
Sci Rep. 2019 Jan 17;9(1):159. (PMID: 30655565)
Sci Rep. 2021 Jul 29;11(1):15470. (PMID: 34326426)
Carbohydr Polym. 2017 May 15;164:145-153. (PMID: 28325311)
Carbohydr Polym. 2014 Oct 13;111:783-7. (PMID: 25037416)
PLoS Pathog. 2013 Jan;9(1):e1003080. (PMID: 23326227)
Nature. 2007 May 3;447(7140):92-6. (PMID: 17450126)
Science. 2004 Jun 11;304(5677):1678-82. (PMID: 15192232)
J Mol Biol. 1978 Apr 5;120(2):167-81. (PMID: 642008)
PLoS One. 2012;7(11):e50381. (PMID: 23185612)
Int J Mol Sci. 2015 Feb 13;16(2):4028-42. (PMID: 25689423)
Sci Rep. 2017 Oct 11;7(1):12963. (PMID: 29021549)
J Biol Chem. 2001 Mar 2;276(9):6770-8. (PMID: 11085997)
Glycobiology. 2015 May;25(5):469-82. (PMID: 25595947)
Cell. 2017 Apr 20;169(3):497-509.e13. (PMID: 28431248)
J Biophys Biochem Cytol. 1957 Sep 25;3(5):669-83. (PMID: 13475384)
Annu Rev Physiol. 2011;73:479-501. (PMID: 21054166)
Cornea. 2009 Jul;28(6):667-72. (PMID: 19512904)
Asian-Australas J Anim Sci. 2016 Jul;29(7):979-86. (PMID: 27282974)
Sci Rep. 2019 Oct 30;9(1):15609. (PMID: 31666642)
FEBS Lett. 2017 Oct;591(20):3310-3318. (PMID: 28833103)
Sci Rep. 2017 Jul 27;7(1):6662. (PMID: 28751762)
Eur J Gastroenterol Hepatol. 2009 Oct;21(10):1119-26. (PMID: 19242357)
BMC Med Genomics. 2013 Oct 11;6:41. (PMID: 24119614)
J Biol Chem. 2009 Jul 17;284(29):19650-8. (PMID: 19435888)
Front Pharmacol. 2011 Jul 25;2:43. (PMID: 21811466)
Sci Rep. 2018 Jan 23;8(1):1461. (PMID: 29362395)
Am J Respir Cell Mol Biol. 2012 Jan;46(1):71-9. (PMID: 21836154)
PLoS One. 2013 Jun 27;8(6):e67399. (PMID: 23826286)
MethodsX. 2020 Apr 18;7:100881. (PMID: 32346528)
Biochem J. 1990 Sep 15;270(3):705-13. (PMID: 2241903)
Am J Respir Crit Care Med. 2005 Dec 15;172(12):1505-9. (PMID: 16179638)
Carbohydr Res. 2007 Dec 28;342(18):2750-6. (PMID: 17889843)
Mar Drugs. 2010 Apr 27;8(5):1482-517. (PMID: 20559485)

Keywords: FACE method; acidic chitinase; block-type chitosan; chitin; chitooligosaccharides; random-type chitosan

0 (Oligosaccharides)
0 (oligochitosan)
9012-76-4 (Chitosan)
EC 3.2.1.14 (Chitinases)

Date Created: 20211113 Date Completed: 20211206 Latest Revision: 20211214

20250114

PMC8587675

10.3390/molecules26216706

34771117