Xylooligosaccharide Production with Low Xylose Release Using Crude Xylanase from Aureobasidium pullulans: Effect of the Enzymatic Hydrolysis Parameters.

Publisher: Humana Press Country of Publication: United States NLM ID: 8208561 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-0291 (Electronic) Linking ISSN: 02732289 NLM ISO Abbreviation: Appl Biochem Biotechnol

Original Publication: Clifton, N.J. : Humana Press, c1981-

Oligosaccharides*/chemistry ; Oligosaccharides*/metabolism ; Glucuronates*/metabolism ; Endo-1,4-beta Xylanases*/metabolism ; Xylose*/metabolism ; Aureobasidium*/metabolism; Hydrolysis ; Hydrogen-Ion Concentration ; Xylans/metabolism ; Fungal Proteins/metabolism ; Fungal Proteins/chemistry ; Temperature

Xylooligosaccharides (XOS) are non-digestible and fermentable oligomers that stand out for their efficient production by enzymatic hydrolysis and beneficial effects on human health. This study aimed to investigate the influence of the main reaction parameters of the beechwood xylan hydrolysis using crude xylanase from Aureobasidium pullulans CCT 1261, thus achieving the maximum XOS production. The effects of temperature (40 to 50 °C), reaction time (12 to 48 h), type of agitation, substrate concentration (1 to 6%, w/v), xylanase loading (100 to 300 U/g xylan), and pH (4.0 to 6.0) on the XOS production were fully evaluated. The most suitable conditions for XOS production included orbital shaking of 180 rpm, 40 °C, and 24 h of reaction. High contents of total XOS (10.1 mg/mL) and XOS with degree of polymerization (DP) of 2-3 (9.7 mg/mL), besides to a high percentage of XOS (99.1%), were obtained at 6% (w/v) of beechwood xylan, xylanase loading of 260 U/g xylan, and pH 6.0. The establishment of the best hydrolysis conditions allowed increasing both the content of total XOS 1.5-fold and the percentage of XOS by 9.4%, when compared to the initial production (6.7 mg/mL and 89.7%, respectively). Thus, this study established an efficient enzymatic hydrolysis process that results in a hydrolysate containing XOS with potential prebiotic character (i.e., rich in XOS with DP 2-3) and low xylose amounts.
(© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

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001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; 423285/2018-1 - 304857/2018-1 Conselho Nacional de Desenvolvimento Científico e Tecnológico

Keywords: Enzymatic hydrolysis; Experimental design; Potential prebiotics; Xylooligomers

0 (Oligosaccharides)
0 (xylooligosaccharide)
0 (Glucuronates)
EC 3.2.1.8 (Endo-1,4-beta Xylanases)
A1TA934AKO (Xylose)
0 (Xylans)
0 (Fungal Proteins)

Aureobasidium pullulans

Date Created: 20210922 Date Completed: 20240724 Latest Revision: 20240828

20240828

10.1007/s12010-021-03658-x

34550500