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Use of human PBMC to analyse the impact of obesity on lipid metabolism and metabolic status: a proof-of-concept pilot study.

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  • معلومة اضافية
    • المصدر:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: London : Nature Publishing Group, copyright 2011-
    • الموضوع:
      Biomarkers*/blood ; Lipid Metabolism*; Leukocytes, Mononuclear/*metabolism ; Obesity/*metabolism; Absorptiometry, Photon ; Adolescent ; Adult ; Body Composition ; Body Weights and Measures ; Disease Susceptibility ; Female ; Humans ; Male ; Middle Aged ; Obesity/etiology ; Overweight/etiology ; Overweight/metabolism ; Pilot Projects ; Principal Component Analysis ; Young Adult
    • نبذة مختصرة :
      Peripheral blood mononuclear cells (PBMC) are widely used as a biomarker source in nutrition/obesity studies because they reflect gene expression profiles of internal tissues. In this pilot proof-of-concept study we analysed in humans if, as we previously suggested in rodents, PBMC could be a surrogate tissue to study overweight/obesity impact on lipid metabolism. Pre-selected key lipid metabolism genes based in our previous preclinical studies were analysed in PBMC of normoglycemic normal-weight (NW), and overweight-obese (OW-OB) subjects before and after a 6-month weight-loss plan. PBMC mRNA levels of CPT1A, FASN and SREBP-1c increased in the OW-OB group, according with what described in liver and adipose tissue of humans with obesity. This altered expression pattern was related to increased adiposity and early signs of metabolic impairment. Greater weight loss and/or metabolic improvement as result of the intervention was related to lower CPT1A, FASN and SREBP-1c gene expression in an adjusted linear mixed-effects regression analysis, although no gene expression recovery was observed when considering mean comparisons. Thus, human PBMC reflect lipid metabolism expression profile of energy homeostatic tissues, and early obesity-related alterations in metabolic at-risk subjects. Further studies are needed to understand PBMC usefulness for analysis of metabolic recovery in weigh management programs.
      (© 2021. The Author(s).)
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    • الرقم المعرف:
      0 (Biomarkers)
    • الموضوع:
      Date Created: 20210916 Date Completed: 20211210 Latest Revision: 20211214
    • الموضوع:
      20240628
    • الرقم المعرف:
      PMC8443582
    • الرقم المعرف:
      10.1038/s41598-021-96981-6
    • الرقم المعرف:
      34526523