Susceptibility and cytokine responses of human neuronal cells to multiple circulating EV-A71 genotypes in India.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Cytokines/*biosynthesis ; Enterovirus A, Human/*pathogenicity ; Hand, Foot and Mouth Disease/*virology ; Neurons/*virology; Acute Disease ; Adult ; Cell Line, Tumor ; Child ; Cytokines/genetics ; Cytopathogenic Effect, Viral ; Disease Outbreaks ; Disease Susceptibility ; Encephalitis, Viral/epidemiology ; Encephalitis, Viral/virology ; Enterovirus A, Human/classification ; Enterovirus A, Human/genetics ; Enterovirus A, Human/isolation & purification ; Female ; Gene Expression Regulation, Viral ; Genotype ; Hand, Foot and Mouth Disease/epidemiology ; Humans ; India/epidemiology ; Male ; Middle Aged ; Neurons/metabolism ; Paraplegia/epidemiology ; Paraplegia/virology ; Viral Tropism

Enterovirus-A71 (EV-A71) associated Hand, foot and mouth disease (HFMD) is a highly contagious viral infection affecting children in Asia-Pacific region and has become a major threat to public health. Although several EV-A71 genotypes (C, D, and G) were isolated in India in recent years, no recognizable outbreak of EV-A71 caused HFMD, Acute Flaccid paralysis (AFP) or encephalitis have been reported so far. It is essential to study the pathogenicity or cell tropism of these Indian isolates in order to understand their tendency to cause disease. We investigated the susceptibility and cytokine responses of indigenous EV-A71 genotypes (D and G) isolated from cases of AFP and genotype C viruses isolated from cases of HFMD and encephalitis, in human cells in-vitro. Although all three EV-A71 genotypes could infect and replicate in human muscle and neuronal cells, the genotype D virus showed a delayed response in human neuronal cells. Quantification of cytokine secretion in response to these isolates followed by confirmation with gene expression assays in human neuronal cells revealed significantly higher secretion of pro-inflammatory cytokines TNF-α IL-8, IL-6, IP-10 (p < 0.001) in G genotype infected cells as compared to pathogenic C genotypes whereas the genotype D virus could not induce any of the inflammatory cytokines. These findings will help to better understand the host response to indigenous EV-A71 genotypes for management of future EV-A71 outbreaks in India, if any.
(© 2021. The Author(s).)

J Virol. 2011 Apr;85(7):3067-76. (PMID: 21228224)
Int J Infect Dis. 2010 Feb;14(2):e141-6. (PMID: 20106698)
Cytokine Growth Factor Rev. 2011 Jun;22(3):121-30. (PMID: 21802343)
J Virol. 2010 Aug;84(16):8051-61. (PMID: 20519382)
PLoS One. 2014 Nov 12;9(11):e112676. (PMID: 25391156)
BMC Infect Dis. 2012 Nov 28;12:327. (PMID: 23191987)
Arch Virol. 1979;60(3-4):329-40. (PMID: 228639)
J Infect Dis. 2003 Aug 15;188(4):564-70. (PMID: 12898444)
J Clin Microbiol. 2002 Aug;40(8):2823-7. (PMID: 12149336)
Indian J Dermatol. 2013 Jan;58(1):78-9. (PMID: 23372222)
J Clin Virol. 2000 Jun;17(1):23-30. (PMID: 10814935)
Emerg Health Threats J. 2013 Sep 10;6:19780. (PMID: 24119538)
PLoS One. 2013 Jun 28;8(6):e67430. (PMID: 23840697)
Clin Infect Dis. 2007 Oct 15;45(8):950-7. (PMID: 17879907)
J Virol. 2009 Jul;83(13):6477-83. (PMID: 19386699)
Front Biosci. 1997 Jan 01;2:d12-26. (PMID: 9159205)
Virulence. 2020 Dec;11(1):537-553. (PMID: 32434419)
Emerg Infect Dis. 2015 Jan;21(1):123-6. (PMID: 25531549)
Sci Rep. 2018 Jan 26;8(1):1658. (PMID: 29374213)
Inflamm Res. 2014 Jul;63(7):549-56. (PMID: 24609754)
Indian J Pediatr. 2005 Jan;72(1):17-21. (PMID: 15684442)
Medicine (Baltimore). 2017 Apr;96(17):e6764. (PMID: 28445307)
Infect Dis (Lond). 2015 Jun;47(6):418-22. (PMID: 25751776)
J Clin Microbiol. 2009 Jul;47(7):2351-2. (PMID: 19439545)
Eur J Med Res. 2018 May 3;23(1):21. (PMID: 29724230)
J Virol. 2012 Apr;86(7):3767-76. (PMID: 22258259)
Emerg Infect Dis. 2012 Nov;18(11):1833-40. (PMID: 23092622)
BMC Infect Dis. 2015 Jun 11;15:225. (PMID: 26058678)
J Virol Methods. 2011 Jun;174(1-2):128-30. (PMID: 21440569)
FEMS Microbiol Rev. 2002 Mar;26(1):91-107. (PMID: 12007645)
Mediators Inflamm. 2020 Jan 31;2020:9273241. (PMID: 32089650)
BMC Infect Dis. 2014 May 07;14:243. (PMID: 24886383)
J Gen Virol. 2013 May;94(Pt 5):1019-1027. (PMID: 23288420)

0 (Cytokines)

Date Created: 20210908 Date Completed: 20211116 Latest Revision: 20211116

20231215

PMC8423732

10.1038/s41598-021-97166-x

34493781