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Susceptibility and cytokine responses of human neuronal cells to multiple circulating EV-A71 genotypes in India.
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- المؤلفون: Mohanty MC;Mohanty MC; Varose SY; Varose SY; Saxena VK; Saxena VK
- المصدر:
Scientific reports [Sci Rep] 2021 Sep 07; Vol. 11 (1), pp. 17751. Date of Electronic Publication: 2021 Sep 07.
- نوع النشر :
Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
- اللغة:
English
- معلومة اضافية
- المصدر:
Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
- بيانات النشر:
Original Publication: London : Nature Publishing Group, copyright 2011-
- الموضوع:
Cytokines/
*biosynthesis ;
Enterovirus A, Human/
*pathogenicity ;
Hand, Foot and Mouth Disease/
*virology ;
Neurons/
*virology;
Acute Disease ;
Adult ;
Cell Line, Tumor ;
Child ;
Cytokines/
genetics ;
Cytopathogenic Effect, Viral ;
Disease Outbreaks ;
Disease Susceptibility ;
Encephalitis, Viral/
epidemiology ;
Encephalitis, Viral/
virology ;
Enterovirus A, Human/
classification ;
Enterovirus A, Human/
genetics ;
Enterovirus A, Human/
isolation & purification ;
Female ;
Gene Expression Regulation, Viral ;
Genotype ;
Hand, Foot and Mouth Disease/
epidemiology ;
Humans ;
India/
epidemiology ;
Male ;
Middle Aged ;
Neurons/
metabolism ;
Paraplegia/
epidemiology ;
Paraplegia/
virology ;
Viral Tropism - نبذة مختصرة :
Enterovirus-A71 (EV-A71) associated Hand, foot and mouth disease (HFMD) is a highly contagious viral infection affecting children in Asia-Pacific region and has become a major threat to public health. Although several EV-A71 genotypes (C, D, and G) were isolated in India in recent years, no recognizable outbreak of EV-A71 caused HFMD, Acute Flaccid paralysis (AFP) or encephalitis have been reported so far. It is essential to study the pathogenicity or cell tropism of these Indian isolates in order to understand their tendency to cause disease. We investigated the susceptibility and cytokine responses of indigenous EV-A71 genotypes (D and G) isolated from cases of AFP and genotype C viruses isolated from cases of HFMD and encephalitis, in human cells in-vitro. Although all three EV-A71 genotypes could infect and replicate in human muscle and neuronal cells, the genotype D virus showed a delayed response in human neuronal cells. Quantification of cytokine secretion in response to these isolates followed by confirmation with gene expression assays in human neuronal cells revealed significantly higher secretion of pro-inflammatory cytokines TNF-α IL-8, IL-6, IP-10 (p < 0.001) in G genotype infected cells as compared to pathogenic C genotypes whereas the genotype D virus could not induce any of the inflammatory cytokines. These findings will help to better understand the host response to indigenous EV-A71 genotypes for management of future EV-A71 outbreaks in India, if any.
(© 2021. The Author(s).)
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- الرقم المعرف:
0 (Cytokines)
- الموضوع:
Date Created: 20210908 Date Completed: 20211116 Latest Revision: 20211116
- الموضوع:
20231215
- الرقم المعرف:
PMC8423732
- الرقم المعرف:
10.1038/s41598-021-97166-x
- الرقم المعرف:
34493781
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