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18β-Glycyrrhetinic acid inhibits the apoptosis of cells infected with rotavirus SA11 via the Fas/FasL pathway.

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  • معلومة اضافية
    • المصدر:
      Publisher: Taylor & Francis Country of Publication: England NLM ID: 9812552 Publication Model: Print Cited Medium: Internet ISSN: 1744-5116 (Electronic) Linking ISSN: 13880209 NLM ISO Abbreviation: Pharm Biol Subsets: MEDLINE
    • بيانات النشر:
      Publication: [London] : Taylor & Francis
      Original Publication: Lisse, the Netherlands : Swets & Zeitlinger, c1998-
    • الموضوع:
      Antiviral Agents/*pharmacology ; Glycyrrhetinic Acid/*analogs & derivatives ; Rotavirus/*drug effects ; Rotavirus Infections/*drug therapy; Animals ; Antiviral Agents/administration & dosage ; Apoptosis/drug effects ; Cell Cycle/drug effects ; Cell Line ; Dose-Response Relationship, Drug ; Fas Ligand Protein/metabolism ; Glycyrrhetinic Acid/administration & dosage ; Glycyrrhetinic Acid/pharmacology ; Haplorhini ; RNA, Messenger/metabolism ; Rotavirus Infections/virology ; fas Receptor/metabolism
    • نبذة مختصرة :
      Context: 18β-Glycyrrhetinic acid (18β-GA), a pentacyclic triterpenoid saponin metabolite of glycyrrhizin, exhibits several biological activities.
      Objective: We investigated the effects of 18β-GA on MA104 cells infected with rotavirus (RV) and its potential mechanism of action.
      Materials and Methods: Cell Counting Kit-8 was used to assess tissue culture infective dose 50 (TCID 50 ) and 50% cellular cytotoxicity (CC 50 ) concentration. MA104 cells infected with RV SA11 were treated with 18β-GA (1, 2, 4, and 8 μg/mL, respectively). Cytopathic effects were observed. The virus inhibition rate, concentration for 50% of maximal effect (EC 50 ), and selection index (SI) were calculated. Cell cycle, cell apoptosis, and mRNA and protein expression related to the Fas/FasL pathway were detected.
      Results: TCID 50 of RV SA11 was 10 -4.47 /100 µL; the CC 50 of 18β-GA on MA104 cells was 86.92 µg/mL. 18β-GA showed significant antiviral activity; EC 50 was 3.14 μg/mL, and SI was 27.68. The ratio of MA104 cells infected with RV SA11 in the G0/G1 phase and the G2/M phase decreased and increased, respectively, after 18β-GA treatment. 18β-GA significantly induced apoptosis in the infected cells. Furthermore, after 18β-GA treatment, the mRNA and protein expression levels of Fas, FasL, caspase 3, and Bcl-2 decreased, whereas the expression levels of Bax increased.
      Discussion and Conclusions: The study demonstrates that 18β-GA may be a promising candidate for the treatment of RV SA11 infection and provides theoretical support for the clinical development of glycyrrhizic acid compounds for the treatment of RV infection.
    • Comments:
      Retraction in: Pharm Biol. 2022 Dec;60(1):2200. doi: 10.1080/13880209.2022.2142339. (PMID: 36345877)
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    • Contributed Indexing:
      Keywords: MA104 cells; cell cycle; rotavirus infection
    • الرقم المعرف:
      0 (Antiviral Agents)
      0 (Fas Ligand Protein)
      0 (RNA, Messenger)
      0 (fas Receptor)
      1449-05-4 (18alpha-glycyrrhetinic acid)
      P540XA09DR (Glycyrrhetinic Acid)
    • الموضوع:
      Date Created: 20210819 Date Completed: 20220106 Latest Revision: 20221108
    • الموضوع:
      20250114
    • الرقم المعرف:
      PMC8382007
    • الرقم المعرف:
      10.1080/13880209.2021.1961821
    • الرقم المعرف:
      34411493