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Genetic engineering of Pseudomonas chlororaphis Lzh-T5 to enhance production of trans-2,3-dihydro-3-hydroxyanthranilic acid.

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  • معلومة اضافية
    • المصدر:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: London : Nature Publishing Group, copyright 2011-
    • الموضوع:
      3-Hydroxyanthranilic Acid/*metabolism ; Pseudomonas chlororaphis/*genetics; 3-Hydroxyanthranilic Acid/chemistry ; Culture Media ; Fermentation ; Ferric Compounds/metabolism ; Gene Knockdown Techniques ; Genes, Bacterial ; Genes, Regulator ; Phenazines/metabolism
    • نبذة مختصرة :
      Trans-2,3-dihydro-3-hydroxyanthranilic acid (DHHA) is a cyclic β-amino acid used for the synthesis of non-natural peptides and chiral materials. And it is an intermediate product of phenazine production in Pseudomonas spp. Lzh-T5 is a P. chlororaphis strain isolated from tomato rhizosphere found in China. It can synthesize three antifungal phenazine compounds. Disruption the phzF gene of P. chlororaphis Lzh-T5 results in DHHA accumulation. Several strategies were used to improve production of DHHA: enhancing the shikimate pathway by overexpression, knocking out negative regulatory genes, and adding metal ions to the medium. In this study, three regulatory genes (psrA, pykF, and rpeA) were disrupted in the genome of P. chlororaphis Lzh-T5, yielding 5.52 g/L of DHHA. When six key genes selected from the shikimate, pentose phosphate, and gluconeogenesis pathways were overexpressed, the yield of DHHA increased to 7.89 g/L. Lastly, a different concentration of Fe 3+ was added to the medium for DHHA fermentation. This genetically engineered strain increased the DHHA production to 10.45 g/L. According to our result, P. chlororaphis Lzh-T5 could be modified as a microbial factory to produce DHHA. This study laid a good foundation for the future industrial production and application of DHHA.
      (© 2021. The Author(s).)
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    • الرقم المعرف:
      0 (Culture Media)
      0 (Ferric Compounds)
      0 (Phenazines)
      0 (phenazine)
      1UQB1BT4OT (3-Hydroxyanthranilic Acid)
    • الموضوع:
      Date Created: 20210813 Date Completed: 20211112 Latest Revision: 20240403
    • الموضوع:
      20250114
    • الرقم المعرف:
      PMC8361184
    • الرقم المعرف:
      10.1038/s41598-021-94674-8
    • الرقم المعرف:
      34385485