Selection of DNA Aptamers for Differentiation of Human Adipose-Derived Mesenchymal Stem Cells from Fibroblasts.

Publisher: Humana Press Country of Publication: United States NLM ID: 8208561 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-0291 (Electronic) Linking ISSN: 02732289 NLM ISO Abbreviation: Appl Biochem Biotechnol Subsets: MEDLINE

Original Publication: Clifton, N.J. : Humana Press, c1981-

Adipose Tissue/*metabolism ; Aptamers, Nucleotide/*pharmacology ; Cell Differentiation/*drug effects ; Fibroblasts/*metabolism ; Mesenchymal Stem Cells/*metabolism; Adipose Tissue/cytology ; Aptamers, Nucleotide/chemistry ; Cells, Cultured ; Fibroblasts/cytology ; Humans ; Mesenchymal Stem Cells/cytology

In recent years, stem cell therapy has shown promise in regenerative medicine. The lack of standardized protocols for cell isolation and differentiation generates conflicting results in this field. Mesenchymal stem cells derived from adipose tissue (ASC) and fibroblasts (FIB) share very similar cell membrane markers. In this context, the distinction of mesenchymal stem cells from fibroblasts has been crucial for safe clinical application of these cells. In the present study, we developed aptamers capable of specifically recognize ASC using the Cell-SELEX technique. We tested the affinity of ASC aptamers compared to dermal FIB. Quantitative PCR was advantageous for the in vitro validation of four candidate aptamers. The binding capabilities of Apta 2 and Apta 42 could not distinguish both cell types. At the same time, Apta 21 and Apta 99 showed a better binding capacity to ASC with dissociation constants (Kd) of 50.46 ± 2.28 nM and 72.71 ± 10.3 nM, respectively. However, Apta 21 showed a Kd of 86.78 ± 9.14 nM when incubated with FIB. Therefore, only Apta 99 showed specificity to detect ASC by total internal reflection microscopy (TIRF). This aptamer is a promising tool for the in vitro identification of ASC. These results will help understand the differences between these two cell types for more specific and precise cell therapies.
(© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Erratum in: Appl Biochem Biotechnol. 2021 Oct 7;:. (PMID: 34618339)

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Keywords: Cell-SELEX; DNA aptamers; Human adipose-derived stem cells; Human dermal fibroblasts; Quantitative PCR assay

0 (Aptamers, Nucleotide)

Date Created: 20210807 Date Completed: 20220117 Latest Revision: 20220212

20231215

10.1007/s12010-021-03618-5

34363139