Variability of the Chemical Composition and Bioactivity between the Essential Oils Isolated from Male and Female Specimens of Hedyosmum racemosum (Ruiz & Pav.) G. Don.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, c1995-

Anti-Bacterial Agents/*chemistry ; Antioxidants/*chemistry ; Cyclohexane Monoterpenes/*chemistry ; Magnoliopsida/*chemistry ; Oils, Volatile/*chemistry; Allylbenzene Derivatives/chemistry ; Allylbenzene Derivatives/isolation & purification ; Anisoles/chemistry ; Anisoles/isolation & purification ; Anti-Bacterial Agents/isolation & purification ; Anti-Bacterial Agents/pharmacology ; Antioxidants/isolation & purification ; Antioxidants/pharmacology ; Arthrodermataceae/drug effects ; Arthrodermataceae/growth & development ; Benzothiazoles/antagonists & inhibitors ; Bicyclic Monoterpenes/chemistry ; Bicyclic Monoterpenes/isolation & purification ; Biphenyl Compounds/antagonists & inhibitors ; Cyclohexane Monoterpenes/isolation & purification ; Ecuador ; Eugenol/analogs & derivatives ; Eugenol/chemistry ; Eugenol/isolation & purification ; Female ; Gram-Negative Bacteria/drug effects ; Gram-Negative Bacteria/growth & development ; Gram-Positive Bacteria/drug effects ; Gram-Positive Bacteria/growth & development ; Humans ; Magnoliopsida/metabolism ; Male ; Microbial Sensitivity Tests ; Picrates/antagonists & inhibitors ; Plant Leaves/chemistry ; Plants, Medicinal ; Sesquiterpenes, Germacrane/chemistry ; Sesquiterpenes, Germacrane/isolation & purification ; Sex Factors ; Sulfonic Acids/antagonists & inhibitors

Hedyosmum racemosum (Ruiz & Pav.) G. is a native species of Ecuador used in traditional medicine for treatment of rheumatism, bronchitis, cold, cough, asthma, bone pain, and stomach pain. In this study, fresh H. racemosum leaves of male and female specimens were collected and subjected to hydrodistillation for the extraction of the essential oil. The chemical composition of male and female essential oil was determined by gas chromatography-gas chromatography equipped with a flame ionization detector and coupled to a mass spectrometer using a non-polar and a polar chromatographic column. The antibacterial activity was assayed against five Gram-positive and two Gram-negative bacteria, and two dermatophytes fungi. The scavenging radical properties of the essential oil were evaluated by DPPH and ABTS assays. The chemical analysis allowed us to identify forty-three compounds that represent more than 98% of the total composition. In the non-polar and polar column, α-phellandrene was the principal constituent in male (28.24 and 25.90%) and female (26.47 and 23.90%) essential oil. Other main compounds were methyl chavicol, germacrene D, methyl eugenol, and α-pinene. Female essential oil presented a strong activity against Klebsiella pneumoniae (ATCC 9997) with an minimum inhibitory concentration (MIC) of 500 μg/mL and a scavenging capacity SC 50 of 800 µg/mL.

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Keywords: Hedyosmum racemosum; Klebsiella pneumoniae; chemical composition; essential oils; α-phellandrene

0 (Allylbenzene Derivatives)
0 (Anisoles)
0 (Anti-Bacterial Agents)
0 (Antioxidants)
0 (Benzothiazoles)
0 (Bicyclic Monoterpenes)
0 (Biphenyl Compounds)
0 (Cyclohexane Monoterpenes)
0 (Oils, Volatile)
0 (Picrates)
0 (Sesquiterpenes, Germacrane)
0 (Sulfonic Acids)
28752-68-3 (2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid)
29T9VA6R7M (methyleugenol)
3T8H1794QW (Eugenol)
49JV13XE39 (alpha phellandrene)
9NIW07V3ET (estragole)
DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
JPF3YI7O34 (alpha-pinene)
V2I9ATG34E (germacrene D)

Date Created: 20210807 Date Completed: 20210914 Latest Revision: 20210914

20250114

PMC8347853

10.3390/molecules26154613

34361766