Myogenetic oligodeoxynucleotide complexed with berberine promotes differentiation of chicken myoblasts.

Publisher: Wiley Country of Publication: Australia NLM ID: 100956805 Publication Model: Print Cited Medium: Internet ISSN: 1740-0929 (Electronic) Linking ISSN: 13443941 NLM ISO Abbreviation: Anim Sci J Subsets: MEDLINE

Publication: Richmond, Vic. : Wiley
Original Publication: Tokyo, Japan : Japanese Society of Zootechnical Science [1999-

Berberine*/pharmacology ; Chickens*/genetics; Animals ; Cell Differentiation ; Muscle Development ; MyoD Protein/genetics ; Myoblasts ; Myogenin/genetics ; Myosin Heavy Chains/genetics ; Oligodeoxyribonucleotides

Myoblasts are myogenic precursors that develop into myotubes during muscle formation. Improving efficiency of myoblast differentiation is important for advancing meat production by domestic animals. We recently identified novel oligodeoxynucleotides (ODNs) termed myogenetic ODNs (myoDNs) that promote the differentiation of mammalian myoblasts. An isoquinoline alkaloid, berberine, forms a complex with one of the myoDNs, iSN04, and enhances its activities. This study investigated the effects of myoDNs on chicken myoblasts to elucidate their species-specific actions. Seven myoDNs (iSN01-iSN07) were found to facilitate the differentiation of chicken myoblasts into myosin heavy chain (MHC)-positive myotubes. The iSN04-berberine complex exhibited a higher myogenetic activity than iSN04 alone, which was shown to enhance the differentiation of myoblasts into myotubes and the upregulation of myogenic gene expression (MyoD, myogenin, MHC, and myomaker). These data indicate that myoDNs promoting chicken myoblast differentiation may be used as potential feed additives in broiler diets.
(© 2021 Japanese Society of Animal Science.)

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H29-3-12 Fund of Nagano Prefecture to Promote Scientific Activity; Ito Foundation; 18K05939 Japan Society for the Promotion of Science; 19J20888 Japan Society for the Promotion of Science; 19K05948 Japan Society for the Promotion of Science; 2018C002 Kieikai Research Foundation; Shinshu Foundation for Promotion of Agricultural and Forest Science

Keywords: berberine; chicken; myoblast; myogenetic oligodeoxynucleotide (myoDN); myogenic differentiation

0 (MyoD Protein)
0 (Myogenin)
0 (Oligodeoxyribonucleotides)
0I8Y3P32UF (Berberine)
EC 3.6.4.1 (Myosin Heavy Chains)

Date Created: 20210726 Date Completed: 20220210 Latest Revision: 20230503

20230503

10.1111/asj.13597

34309956