A green and facile approach to a graphene-based peroxidase-like nanozyme and its application in sensitive colorimetric detection of L-cysteine.

Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101134327 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-2650 (Electronic) Linking ISSN: 16182642 NLM ISO Abbreviation: Anal Bioanal Chem Subsets: MEDLINE

Original Publication: Heidelberg : Springer-Verlag, 2002-

Green Chemistry Technology*; Colorimetry/*methods ; Cysteine/*analysis ; Graphite/*chemistry ; Peroxidase/*chemistry; Limit of Detection

A facile and green approach to the preparation of peroxidase-like nanozymes by reducing and functionalizing graphene oxide (rGO) with Ganoderma polysaccharide (GP) has been achieved in this work. Our results showed that the as-fabricated nanozyme, namely rGO-GP, possessed the excellent property of simulating peroxidase with higher catalytic activity compared with GO or rGO obtained by using chitosan, which may be due to the better dispersion of rGO-GP in the solution. Steady-state kinetics studies further showed that the catalytic process conformed to Michaelis-Menten equation and ping-pong mechanism. Benefiting from the excellent peroxidase property of rGO-GP, we have also successfully established a highly sensitive and selective colorimetric detection approach to trace detection of L-cysteine (L-Cys). The limit of detection (LOD) of L-cysteine is 0.1 μM and the linear detection range is 2-30 μM. Furthermore, the nanozyme was successfully applied for detecting L-cysteine in serum. This work therefore demonstrates the advantages of rGO-GP as an effective nanozyme in both its green synthesis and detecting application.

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Keywords: Ganoderma polysaccharide (GP); Graphene oxide (GO); Green synthesis; L-Cysteine; Nanozymes; Reduced graphene oxide (rGO)

7782-42-5 (Graphite)
EC 1.11.1.7 (Peroxidase)
K848JZ4886 (Cysteine)

Date Created: 20210507 Date Completed: 20210914 Latest Revision: 20210914

20250114

10.1007/s00216-021-03352-1

33961104